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Updated: Jul 27, 2025

Optimization and Comparative Analysis of Plant Organellar DNA Enrichment Methods Suitable for Next-generation Sequencing
Published on: July 28, 2017
Analysis of the complete plastomes of
Xi Wu1, Mei Jiang1, Michelle Liu2
1Key Laboratory of Bioactive Substances and Resource Utilization of Chinese Herbal Medicine from the Ministry of Education, Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing, People's Republic of China.
Bidens pilosa plastome sequencing revealed high genetic diversity, suggesting potential misidentifications in medicinal products. Accurate species identification is crucial for ensuring the quality of herbal medicines.
Area of Science:
- Genomics
- Plant Science
- Ethnobotany
Background:
- Bidens pilosa L. is a widely used plant in traditional medicine and food.
- Accurate identification of medicinal plants is vital for efficacy and safety.
Purpose of the Study:
- To sequence, assemble, and characterize the complete plastome of Bidens pilosa.
- To investigate the genetic diversity and phylogenetic relationships within Bidens pilosa populations.
Main Methods:
- Whole-genome sequencing and assembly of the Bidens pilosa plastome.
- Bioinformatic analysis of gene content and structure.
- Phylogenetic analysis using K2P distance and molecular markers.
Main Results:
- The complete Bidens pilosa plastome is 150,524 bp with a typical quadripartite structure, containing 128 genes.
- Phylogenetic analyses indicated high genetic diversity and separation into three clades, challenging the monophyletic nature of the studied samples.
- Genetic markers confirmed the observed diversity and potential for misidentification.
Conclusions:
- The study highlights significant genetic diversity within Bidens pilosa, necessitating careful species identification.
- Correct identification of medicinal plant materials is essential for quality control and therapeutic consistency.
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