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Novel Sequence Discovery by Subtractive Genomics
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The complete chloroplast genome of

Li-Jun Zhang1, Ying Liu1, Jian-Jun Shi1

  • 1Qinghai University, Qinghai Academy of Animal and Veterinary Sciences, Qinghai Provincial Key Laboratory of Adaptive Management on Alpine Grassland, Key Laboratory of Superior Forage Germplasm in the Qinghai-Tibetan Plateau, Xining, China.

Mitochondrial DNA. Part B, Resources
|June 5, 2023
PubMed
Summary

The complete chloroplast genome of Corethrodendron multijugum, a medicinal plant, was sequenced. Phylogenetic analysis shows it is closely related to Hedysarum petrovii, offering valuable genetic insights.

Keywords:
Corethrodendron multijugumcomplete chloroplast genomephylogenetic analysis

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Area of Science:

  • Plant genomics
  • Molecular evolution
  • Medicinal botany

Background:

  • Corethrodendron multijugum (Maxim.) (Fabaceae: Corethrodendron), also known as Hedysarum multijugum, is a significant medicinal plant in traditional Chinese medicine.
  • Understanding the genetic diversity and evolutionary relationships within Fabaceae species is crucial for their conservation and utilization.

Purpose of the Study:

  • To sequence and annotate the complete chloroplast genome of Corethrodendron multijugum.
  • To investigate the phylogenetic position of C. multijugum within the Fabaceae family.

Main Methods:

  • Whole chloroplast genome sequencing using the Illumina HiSeq 2500 platform.
  • Bioinformatic annotation of the chloroplast genome, identifying protein-coding genes (PCGs), transfer RNA genes (tRNAs), and ribosomal RNA unit genes (rRNAs).
  • Phylogenetic analysis to determine evolutionary relationships.

Main Results:

  • The complete chloroplast genome of C. multijugum was successfully sequenced and annotated, measuring 122,994 bp.
  • The genome encodes 110 genes: 76 PCGs, 30 tRNAs, and 4 rRNAs, with a G+C content of 34.5%.
  • Phylogenetic analysis indicated that C. multijugum is most closely related to Hedysarum petrovii.

Conclusions:

  • This study provides the first complete chloroplast genome sequence for Corethrodendron multijugum.
  • The findings offer essential genetic resources for further research into the Corethrodendron genus and Fabaceae phylogeny.
  • The close phylogenetic relationship with Hedysarum petrovii deepens our understanding of legume evolution.