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M-MDSC

Arpa Aintablian1, Sandra Strozniak1, Marion Heuer1

  • 1Institute for Virology and Immunobiology, University of Würzburg, Würzburg, Germany.

Frontiers in Immunology
|June 5, 2023
PubMed
Summary
This summary is machine-generated.

Interleukin-3 (IL-3) generates monocytic myeloid-derived suppressor cells (M-MDSC) in vitro, similar to GM-CSF. These IL-3-generated M-MDSC exhibit higher arginase 1 (Arg1) expression and enhanced Arg1-mediated immune suppression.

Keywords:
GM-CSFIL-3bone marrowin vitro culturemyeloid-derived suppressor cells (MDSC)protocol

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Area of Science:

  • Immunology
  • Cell Biology

Background:

  • Myeloid-derived suppressor cells (MDSC) are key regulators of immune responses.
  • Inducible nitric oxide synthase (iNOS) and arginase 1 (Arg1) are critical MDSC effector functions.
  • Granulocyte-macrophage colony-stimulating factor (GM-CSF) promotes MDSC development, but IL-3's role is less understood.

Purpose of the Study:

  • To investigate the potential of IL-3 in generating monocytic MDSC (M-MDSC) in vitro.
  • To compare IL-3-generated M-MDSC with those generated by GM-CSF.

Main Methods:

  • Murine bone marrow (BM) cells were cultured in vitro with IL-3 or GM-CSF for 3 days.
  • Phenotypic analysis using flow cytometry identified M-MDSC and other cell types.
  • Functional assays assessed suppressor activity using iNOS and Arg1 inhibitors after LPS + IFN-γ activation.

Main Results:

  • IL-3, like GM-CSF, generated M-MDSC with comparable phenotype and yield.
  • IL-3-generated M-MDSC showed lower TNF, IL-1β, and IL-10 production but higher Arg1 expression.
  • IL-3-generated M-MDSC exhibited increased Arg1-dependent suppressor activity.

Conclusions:

  • IL-3 can generate M-MDSC in vitro, mirroring GM-CSF's capacity.
  • IL-3-induced M-MDSC possess distinct functional properties, notably enhanced Arg1 expression and activity.
  • This finding provides a new in vitro model for studying IL-3's role in MDSC biology.