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Rong Li1, Sheng Xu2, Bei Li1

  • 1Key Laboratory of Molecular Biophysics of the Ministry of Education, Hubei Key Laboratory of Bioinformatics and Molecular Imaging, Department of Biotechnology, College of Life Science and Technology, Huazhong University of Science and Technology, No. 1037 Luoyu Road, Hongshan District, Wuhan 430074, Hubei, China. zhiliu@hust.edu.cn.

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|June 6, 2023
PubMed
Summary
This summary is machine-generated.

Refractory constipation involves gut microbiota dysbiosis. A specific strain, Ruminococcus gnavus, found in patient feces, effectively improved constipation symptoms in mice, suggesting a potential microbial treatment.

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Area of Science:

  • Microbiology
  • Gastroenterology
  • Medical Science

Background:

  • Refractory constipation, a severe form, has an unknown cause and significantly impacts patients' physical and psychological well-being.
  • Constipation is linked to gut microbiota dysbiosis, with distinct differences observed between healthy individuals and patients.
  • Accumulating evidence suggests the gut microbiome plays a crucial role in the pathophysiology of constipation.

Purpose of the Study:

  • To investigate the differences in gut microbiota composition between fresh and accumulated feces in patients with refractory constipation.
  • To evaluate the therapeutic potential of specific gut microbes, particularly indigenous strains, for treating refractory constipation.
  • To explore the mechanisms by which gut microbes influence intestinal function and behavior in constipation.

Main Methods:

  • Analysis of gut microbiota composition in fresh and accumulated fecal samples from refractory constipation patients.
  • Establishment of a loperamide-induced constipation mouse model to assess the effects of fecal microbiota transplantation.
  • Oral administration of the identified indigenous strain, Ruminococcus gnavus, to constipated mice.

Main Results:

  • Significant differences in gut microbiota composition were found between fresh and accumulated feces of refractory constipation patients.
  • Fresh feces from patients alleviated constipation symptoms in mice, while old feces exacerbated them, mirroring effects of healthy donor feces.
  • Oral Ruminococcus gnavus administration effectively improved constipation and stress-related behaviors in mice, suggesting a therapeutic role.

Conclusions:

  • Gut microbe intervention using indigenous strains like Ruminococcus gnavus shows promise for treating refractory constipation.
  • Ruminococcus gnavus may exert its beneficial effects by regulating genes like muc2, c-kit, and sert, and controlling somatostatin and motilin production.
  • Targeting gut microbiota represents a potential alternative therapeutic strategy for patients suffering from severe constipation.