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Updated: Jul 27, 2025

A Microscopic Phenotypic Assay for the Quantification of Intracellular Mycobacteria Adapted for High-throughput/High-content Screening

A Microscopic Phenotypic Assay for the Quantification of Intracellular Mycobacteria Adapted for High-throughput/High-content Screening

Published on: January 17, 2014

High-throughput functional genomics: A (myco)bacterial perspective.

Kristy R Winkler¹, Valerie Mizrahi^1,2, Digby F Warner^1,2

¹Molecular Mycobacteriology Research Unit and DSI/NRF Centre of Excellence for Biomedical TB Research, Department of Pathology and Institute of Infectious Disease and Molecular Medicine, University of Cape Town, Rondebosch, South Africa.

Molecular Microbiology

|June 6, 2023

Summary

This summary is machine-generated.

High-throughput functional genomics accelerates bacterial gene discovery. Techniques like transposon mutagenesis and CRISPR interference are crucial for understanding Mycobacterium tuberculosis pathogenicity and developing new drugs.

Keywords:

Mycobacterium tuberculosis CRISPRi Tn-seq TraSH transposon mutagenesis

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Sample Preparation of Mycobacterium tuberculosis Extracts for Nuclear Magnetic Resonance Metabolomic Studies

Sample Preparation of Mycobacterium tuberculosis Extracts for Nuclear Magnetic Resonance Metabolomic Studies

Published on: September 3, 2012

Area of Science:

Microbiology
Genomics
Molecular Biology

Background:

Genomic sequencing provides vast data, but functional validation lags.
Mycobacterium tuberculosis has many genes with unknown functions, hindering drug development.
Efficient methods for large-scale functional genomics are needed.

Purpose of the Study:

To review the evolution of bacterial high-throughput functional genomics.
To highlight transposon-based mutagenesis and CRISPR interference.
To focus on applications for Mycobacterium tuberculosis research and drug discovery.

Main Methods:

Review of transposon (Tn)-based mutagenesis and arrayed mutant libraries.
Analysis of CRISPR interference for large-scale gene function probing.
Contextualizing methods within mycobacterial functional genomics.

Main Results:

Transposon mutagenesis and CRISPR interference are key scalable techniques.
These methods offer insights into bacterial gene function.
Functional genomics can reveal M. tuberculosis pathogenicity and drug targets.

Conclusions:

Accelerating functional genomics is vital for understanding bacterial pathogens.
Mycobacterial functional genomics can guide new drug and regimen development.
Future approaches are needed to fully elucidate M. tuberculosis biology.