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Related Concept Videos

Biofilms01:29

Biofilms

61
Biofilms are complex communities of microorganisms encased in a self-produced extracellular polysaccharide matrix attached to surfaces. These microbial consortia can include single or multiple species, providing enhanced survival benefits by forming organized, multilayered structures.The formation of biofilms occurs through four key stages: attachment, colonization, development, and dispersal.During attachment, free-swimming planktonic cells adhere to a surface, often facilitated by...
61
Biological Methods for Microbial Control01:28

Biological Methods for Microbial Control

145
Biological agents offer an effective means of controlling microbial growth by leveraging natural processes like predation, competition, and the secretion of antimicrobial substances.Predatory bacteria such as Bdellovibrio species target and kill pathogens like Salmonella and E. coli. They are widely used in poultry farms to control infections. Myxococcus species help combat plant-pathogenic fungi. These naturally occurring predators serve as eco-friendly alternatives to chemical pesticides and...
145

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Daily Phototherapy with Red Light to Regulate Candida albicans Biofilm Growth
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Oral Biofilm Cryotherapy as a Novel Ecological Modulation Approach.

N Zayed1,2, J Ghesquière3, N H N Kamarudin4

  • 1Department of Oral Health Sciences, University of Leuven (KU Leuven), Leuven, Belgium.

Journal of Dental Research
|June 6, 2023
PubMed
Summary

Oral cryotherapy effectively reduces oral biofilm load and alters its structure, selectively targeting pathogenic bacteria while preserving beneficial ones. This novel approach shows promise for managing oral biofilms without antimicrobials.

Keywords:
antimicrobial resistancebiofilms managementextracellular matrixmicrobial ecologyoral hygienescanning electron microscopy

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Area of Science:

  • Dentistry
  • Oral Microbiology
  • Biotechnology

Background:

  • Oral cryotherapy is a safe, simple, and cost-effective dental treatment for oral lesions.
  • Its impact on oral biofilms, crucial in oral health, remains unexplored.

Purpose of the Study:

  • To investigate the effects of cryotherapy on in vitro oral biofilms.
  • To assess cryotherapy's potential in modulating oral biofilm ecology.

Main Methods:

  • In vitro multispecies oral biofilms were established on hydroxyapatite discs in symbiotic and dysbiotic states.
  • Biofilms were treated with CryoPen X+ and analyzed immediately or after 24h reincubation using CLSM, SEM, and v-qPCR.

Main Results:

  • Cryotherapy significantly reduced biofilm load, with increased reduction after multiple cycles.
  • While bacterial load recovered within 24h, structural and compositional changes persisted.
  • Pathogenic species incidence decreased significantly in treated biofilms compared to controls.

Conclusions:

  • Spray cryotherapy presents a novel strategy for oral biofilm control.
  • It selectively targets pathobionts, promotes a symbiotic biofilm state, and may prevent dysbiosis without antiseptics/antimicrobials.