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In Vivo Inhibition of MicroRNA to Decrease Tumor Growth in Mice
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MiR-138-5p Inhibits Thyroid Cancer Cell Growth and Stemness by Targeting TRPC5/Wnt/β-Catenin Pathway

Bo Huang1, YiChao Zhang1, Peng Sun1

  • 1Department of General Surgery, The First Affiliated Hospital of Jinan University, No.613, Huangpu Street, Guangzhou, 510000, China.

Molecular Biotechnology
|June 6, 2023
PubMed

Insights

MicroRNA-138-5p suppresses thyroid cancer (TC) cell growth and stemness by targeting TRPC5. This microRNA inhibits proliferation and stemness, promoting apoptosis, and impacting the Wnt/β-catenin pathway in TC progression.

Area of Science:

  • Oncology
  • Molecular Biology
  • Gene Regulation

Background:

  • MicroRNAs are crucial in cancer development, with miR-138-5p showing altered expression in thyroid cancer (TC).
  • The precise role and molecular mechanisms of miR-138-5p in TC progression require further investigation.

Purpose of the Study:

  • To elucidate the function of miR-138-5p in thyroid cancer (TC) progression.
  • To investigate the molecular mechanism underlying miR-138-5p's role in TC, focusing on its interaction with TRPC5 and the Wnt/β-catenin pathway.

Main Methods:

  • Quantitative real-time PCR and Western blot analysis to measure gene and protein expression.
  • Dual-luciferase reporter assay to confirm the interaction between miR-138-5p and TRPC5.
  • Cell-based assays (colony formation, sphere formation, flow cytometry) to assess proliferation, stemness, and apoptosis.

Main Results:

  • miR-138-5p directly targets TRPC5, with inverse expression correlation in TC tissues.
  • miR-138-5p inhibits TC cell proliferation and stemness, while enhancing gemcitabine-induced apoptosis.
  • TRPC5 overexpression reverses the effects of miR-138-5p on cell behavior and Wnt/β-catenin pathway activity.

Conclusions:

  • miR-138-5p acts as a tumor suppressor in thyroid cancer by inhibiting cell growth and stemness.
  • The miR-138-5p/TRPC5/Wnt/β-catenin axis is a key regulatory pathway in TC progression.
  • These findings offer insights into the potential therapeutic role of miR-138-5p in thyroid cancer treatment.
Keywords:
TRPC5Thyroid cancerWnt/β-catenin pathwaymiR-138-5p

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