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Atomic Force Microscopy

Atomic Force Microscopy

Atomic force microscopy (AFM) is a type of scanning probe microscopy that can analyze topographic details of various specimens like ceramics, glass, polymers, and biological samples. AFM offers over 1000 times more resolution than the optical imaging system. Images generated from AFM are three-dimensional surface profiles, offering an advantage over the flat, two-dimensional images from other imaging techniques.
The AFM Probe
The probe is regarded as the heart of any AFM setup and comprises the...

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Utilization of Plasmonic and Photonic Crystal Nanostructures for Enhanced Micro- and Nanoparticle Manipulation

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Polyhedral Au Nanoparticle/MoO

Lin Zhao¹, Tiantian Li¹, Xinlin Xu¹

¹Key Laboratory of Optic-Electric Sensing and Analytical Chemistry for Life Science, Ministry of Education, Shandong Key Laboratory of Biochemical Analysis, and College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao 266042, P. R. China.

Analytical Chemistry

|June 6, 2023

Summary

A novel dual-mode biosensor combines surface-enhanced Raman scattering (SERS) and electrochemistry (EC) for highly sensitive microRNA (miRNA) detection. This advanced platform demonstrates excellent accuracy in biological samples, paving the way for improved clinical diagnostics.

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Published on: September 27, 2011

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High Resolution Physical Characterization of Single Metallic Nanoparticles

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Area of Science:

Nanomaterials Science
Biosensing Technology
Analytical Chemistry

Background:

MicroRNA (miRNA) detection is crucial for disease diagnosis and understanding biological processes.
Existing biosensors often face limitations in sensitivity, selectivity, and complex sample analysis.
Developing novel platforms for accurate and sensitive miRNA quantification remains a significant challenge.

Purpose of the Study:

To construct a highly sensitive dual-mode biosensor for microRNA detection.
To leverage a unique 3D/2D heterojunction and DNA amplification for enhanced sensing performance.
To evaluate the biosensor's efficacy in complex biological matrices like human serum.

Main Methods:

Fabrication of a polyhedral Au nanoparticle/MoOx nanosheet heterojunction (PAMS HJ) substrate.
Utilizing target-triggered nonenzyme cascade autocatalytic DNA amplification (CADA) circuit.
Employing both surface-enhanced Raman scattering (SERS) and electrochemical (EC) detection modes.

Main Results:

The PAMS HJ substrate exhibited synergistic electromagnetic and chemical enhancements, achieving a SERS enhancement factor (EF) of 4.2 × 10^9.
The dual-mode biosensor achieved ultra-low detection limits for miRNA-21: 0.22 aM (SERS) and 2.69 aM (EC).
The platform demonstrated excellent anti-interference capabilities and high accuracy in analyzing miRNA-21 in human serum and cell lysates.

Conclusions:

The developed SERS-EC dual-mode biosensor offers superior sensitivity and selectivity for miRNA detection.
The combination of advanced nanomaterials and DNA amplification strategies significantly enhances biosensing performance.
This novel platform holds significant potential for reliable clinical analysis and advanced biosensing applications.