Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The Subgingival Microbiome Relationship to Periodontal Disease in Older Women.

Journal of dental research·2019
Same author

Cholera and Fever Poisons.

The Indian medical gazette·2017
Same author

Vitamin D Status and Prevalent Early Age-Related Macular Degeneration in African Americans and Caucasians: The Atherosclerosis Risk in Communities (ARIC) Study.

The journal of nutrition, health & aging·2017
Same author

A mutant affecting the crystal cells inDrosophila melanogaster.

Wilhelm Roux's archives of developmental biology·2017
Same author

Developmental analysis of a temperature-sensitive melanotic tumor mutant inDrosophila melanogaster.

Wilhelm Roux's archives of developmental biology·2017
Same author

Hemocyte responses to implanted tissues inDrosophila melanogaster larvae.

Wilhelm Roux's archives of developmental biology·2017

Related Experiment Video

Updated: Jul 27, 2025

Drosophila Embryo Preparation and Microinjection for Live Cell Microscopy Performed using an Automated High Content Analyzer
05:52

Drosophila Embryo Preparation and Microinjection for Live Cell Microscopy Performed using an Automated High Content Analyzer

Published on: January 19, 2021

8.7K

THE SURFACE FEATURES OF DROSOPHILA EMBRYONIC CELL LINES.

T M Rizki1, Rose M Rizki1, C A Andrews1

  • 1Division of Biological Sciences, The University of Michigan, Ann Arbor, Michigan 48109, U.S.A.

Development, Growth & Differentiation
|June 7, 2023
PubMed
Summary

Researchers investigated Schneider

Area of Science:

  • Cell Biology
  • Developmental Biology
  • Biochemistry

Background:

  • Schneider's cell lines are widely used models derived from embryonic Drosophila melanogaster.
  • Understanding cellular heterogeneity within these lines is crucial for accurate experimental interpretation.

Purpose of the Study:

  • To characterize and differentiate three Schneider's cell lines (1, 2, and 3) from Drosophila melanogaster.
  • To identify distinct molecular and morphological features among the cell lines.

Main Methods:

  • Scanning electron microscopy (SEM) for surface morphology.
  • Histochemical staining for periodic acid-Schiff (PAS) positive material.
  • SDS polyacrylamide disc gel electrophoresis for protein analysis.

More Related Videos

Dissection and Live-Imaging of the Late Embryonic Drosophila Gonad
09:08

Dissection and Live-Imaging of the Late Embryonic Drosophila Gonad

Published on: October 17, 2020

3.3K
Imaging and Analysis of Tissue Orientation and Growth Dynamics in the Developing Drosophila Epithelia During Pupal Stages
08:25

Imaging and Analysis of Tissue Orientation and Growth Dynamics in the Developing Drosophila Epithelia During Pupal Stages

Published on: June 2, 2020

9.5K

Related Experiment Videos

Last Updated: Jul 27, 2025

Drosophila Embryo Preparation and Microinjection for Live Cell Microscopy Performed using an Automated High Content Analyzer
05:52

Drosophila Embryo Preparation and Microinjection for Live Cell Microscopy Performed using an Automated High Content Analyzer

Published on: January 19, 2021

8.7K
Dissection and Live-Imaging of the Late Embryonic Drosophila Gonad
09:08

Dissection and Live-Imaging of the Late Embryonic Drosophila Gonad

Published on: October 17, 2020

3.3K
Imaging and Analysis of Tissue Orientation and Growth Dynamics in the Developing Drosophila Epithelia During Pupal Stages
08:25

Imaging and Analysis of Tissue Orientation and Growth Dynamics in the Developing Drosophila Epithelia During Pupal Stages

Published on: June 2, 2020

9.5K

Main Results:

  • SEM revealed distinct age-dependent surface features across the cell lines.
  • Significant differences in the amount and localization of PAS-positive, α-amylase-sensitive material were observed.
  • Line 1 cells contained PAS+ material in vesicles, while lines 2 and 3 showed minimal amounts; electrophoresis detected PAS+ material in line 2 (soluble) and line 3 (pellet) but not line 1.

Conclusions:

  • Schneider's cell lines 1, 2, and 3 exhibit unique morphological and biochemical characteristics.
  • These distinctions, particularly in PAS+ material distribution, highlight cellular heterogeneity and potential functional differences.
  • The findings underscore the importance of considering cell line-specific properties in Drosophila research.