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Updated: Jul 27, 2025

Light-mediated Reversible Modulation of the Mitogen-activated Protein Kinase Pathway during Cell Differentiation and Xenopus Embryonic Development
09:32

Light-mediated Reversible Modulation of the Mitogen-activated Protein Kinase Pathway during Cell Differentiation and Xenopus Embryonic Development

Published on: June 15, 2017

8.8K

Multiple roles of the mitogen-activated protein kinase kinase/mitogen-activated protein kinase cascade in Xenopus

Hidetaka Kosako1, Yukiko Gotoh1, Eisuke Nishida1

  • 1Department of Genetics and Molecular Biology, Institute for Virus Research, Kyoto University, Sakyo-ku, Kyoto 606-01, Japan.

Development, Growth & Differentiation
|June 7, 2023
PubMed

Insights

Mitogen-activated protein kinase (MAPK) signaling is crucial for cell development. This pathway, involving MAPK kinase (MAPKK), regulates key processes in Xenopus oocytes and embryos.

Area of Science:

  • Cell Biology
  • Developmental Biology
  • Molecular Signaling

Background:

  • Mitogen-activated protein kinase (MAPK) is a serine/threonine protein kinase.
  • MAPK is activated by growth factors and tumor promoters in mammalian cells.
  • The MAPK cascade, including MAPK kinase (MAPKK), transmits extracellular signals.

Purpose of the Study:

  • Investigate the role of the MAPKK/MAPK cascade in Xenopus oocyte and embryo development.
  • Understand signal transduction during meiotic maturation.

Main Methods:

  • Utilized neutralizing antibodies against MAPKK and MAPK phosphatases.
  • Employed constitutively active MAPKK and MAPK constructs.
  • Studied developmental processes in Xenopus oocytes and embryos.

Main Results:

  • Demonstrated activation of MAPKK and MAPK during meiotic maturation in Xenopus.
  • Confirmed the crucial role of the MAPKK/MAPK cascade in oocyte and embryo development.

Conclusions:

  • The MAPKK/MAPK cascade is essential for Xenopus oocyte maturation and embryonic development.
  • This signaling pathway plays a vital role in transmitting developmental signals.
Keywords:
Xenopus laeviskinase cascademesoderm inductionmitogen-activated protein kinaseoocyte maturation

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