Related Experiment Video

Updated: Jul 27, 2025

Fluorescent Calcium Imaging and Subsequent In Situ Hybridization for Neuronal Precursor Characterization in Xenopus laevis

Fluorescent Calcium Imaging and Subsequent In Situ Hybridization for Neuronal Precursor Characterization in Xenopus laevis

Published on: February 18, 2020

Ultrastructural Localization of Calcium in the Presumptive Ectodermal Cells in Gastrulae of the Newt, Cynops

Shinji Komazaki¹

¹Department of Anatomy, Saitama Medical School, Moroyama-cho, Iruma-gun, Saitama 350-04, Japan.

Development, Growth & Differentiation

|June 7, 2023

Abstract:

The ultrastructural localization of calcium in the presumptive ectodermal cells of gastrulae of the newt, Cynops pyrrhogaster, was examined by cytochemical methods and X-ray microanalysis (XMA). The cells were fixed with solutions that contained potassium oxalate, potassium ferricyanide and potassium pyroantimonate to preserve the localization of intracellular calcium in situ and for the analysis of electron density due to calcium. Electron-dense deposits associated with the localization of calcium were observed under the electron microscope. Specificially, pigment granules, round vacuoles, endoplasmic reticulum and mitochondria as well as the extracellular matrix were observed to contain calcium. In addition, XMA clearly demonstrated the localization of calcium in all of these electron-dense organelles and yolk granules.

Keywords:

(calcium localization X-ray microanalysis amphibian gastrula cytochemistry ectoderm cell

More Related Videos

Polarized Translocation of Fluorescent Proteins in Xenopus Ectoderm in Response to Wnt Signaling

Polarized Translocation of Fluorescent Proteins in Xenopus Ectoderm in Response to Wnt Signaling

Published on: May 26, 2011

Imaging Calcium in Drosophila at Egg Activation

Imaging Calcium in Drosophila at Egg Activation

Published on: August 6, 2016

Related Concept Videos

Gastrulation

Gastrulation

Gastrulation establishes the three primary tissues of an embryo: the ectoderm, mesoderm, and endoderm. This developmental process relies on a series of intricate cellular movements, which in humans transforms a flat, “bilaminar disc” composed of two cell sheets into a three-tiered structure. In the resulting embryo, the endoderm serves as the bottom layer, and stacked directly above it is the intermediate mesoderm, and then the uppermost ectoderm. Respectively, these tissue strata...

Articles linked to this work by shared authors, journal, and citation graph.

TRIC-A Loss Sensitizes the Heart to β-Adrenergic Stress and Drives Cardiomyocyte Death and Fibrosis.

Biomolecules·2026

Atypical cell death and insufficient matrix organization in long-bone growth plates from Tric-b-knockout mice.

Cell death & disease·2023

Accumulation and Distribution of Extracellular Matrix as Revealed by Scanning Electron Microscopy in Freeze-Dried Newt Embryos Before and During Gastrulation: (extracellular matrix/freeze-drying/SEM/newt embryo).

Development, growth & differentiation·2023

Morphological Changes of the Inner Surface of the Blastocoelic Wall before and during Gastrulation in the Newt, Cynops pyrrhogaster: (amphibia-newt/embryogenesis/blastocoelic wall/cell surface/morphological changes).

Development, growth & differentiation·2023

Movement of an Epithelial Layer Isolated from Early Embryos of the Newt, Cynops pyrrhogaster I. Development of Folding Movement of the Blastocoelic Wall Isolated from Embryos before and during Gastrulation: (blastocoelic wall/folding movement/submembranous microfilaments layer/amphibian embryos).

Development, growth & differentiation·2023

Movement of an Epithelial Layer Isolated from Early Embryos of the Newt, Cynops pyrrhogaster II. Formation of a Blastoporal Groove and Archenteron in a Superficial Epithelial Layer Isolated from Initial Gastrula: (superficial epithelial layer/morphogenetic movement/amphibian gastrulation).

Development, growth & differentiation·2023

See all related articles