INTERACTIONS OF 1-METHYLADENINE AND THE SURFACE OF STARFISH OOCYTE

Three metabolic inhibitors, mycostatin, concanavalin A (Con A) and cytochalasin B (CB) were used to study the interactions between l-methyladenine (1-MA) and the starfish oocyte surface leading to germinal vesicle breakdown (GVB). Mycostatin and Con A had no obvious effects on GVB. CB did not inhibit, but did delay GVB. This delaying effect was interpreted as having multiple 1-MA reactive "sites" on the surface. The results also suggested that not all of them were needed to react with 1-MA to bring about GVB.