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Cellular src Gene Expression Associated with Lentoidogenesis in Transdifferentiating Cultures.: (pp60
Khalid A Tobal1, David K Ellis1, David I Depomerai1
1Department of Zollogy, University of Nottingham, Nottingham NG7 2RD. U.K.
Development, Growth & Differentiation
|June 7, 2023
Summary
Elevated pp60c-src kinase activity is linked to neuroretinal cell transdifferentiation into lens cells. However, c-src oncogene expression does not directly trigger this cellular transformation.
Area of Science:
- Cell Biology
- Developmental Biology
- Molecular Biology
Background:
- Elevated pp60c-src kinase activity is observed during chick embryo neuroretinal (NR) cell transdifferentiation into lens cells in vitro.
- pp60c-src protein is primarily localized in lentoid bodies in permissive cultures.
- pp60c-src expression is low in non-permissive cultures, where lentoid formation is inhibited by high glucose or medium 199.
Purpose of the Study:
- To investigate the role of pp60c-src in the transdifferentiation of NR cells.
- To determine if c-src oncogene expression directly signals transdifferentiation.
- To examine the influence of high glucose and medium 199 on lentoid body formation and associated protein expression.
Main Methods:
- Culturing glial-enriched and tapetal cells in different media (FH, FHG, medium 199).
- Immunological detection and quantification of pp60c-src protein and transcripts.
- Analysis of delta crystallin expression as a marker for lens cell differentiation.
- Utilizing Rous Sarcoma Virus (RSV) variants to study the effect of v-src and c-src expression.
Main Results:
- Glial-enriched cultures form sparse lentoid bodies with increased delta crystallin and pp60c-src expression, but this is limited in high glucose conditions.
- Tapetal cell cultures show higher pp60c-src levels during transdifferentiation compared to redifferentiated or dedifferentiated cells.
- v-src expression inhibits delta crystallin accumulation, while a c-src-substituted RSV variant has minimal impact on NR transdifferentiation.
Conclusions:
- High glucose partially blocks transdifferentiation through neuronal influences, but does not completely inhibit early stages.
- pp60c-src expression correlates with transdifferentiation but does not appear to be the direct signaling molecule initiating the process.
- c-src oncogene expression is not a direct signal for neuroretinal cell transdifferentiation into lens cells.
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