CHANGES IN THE ELECTROPHORETIC MOBILITY OF PRESUMPTIVE ECTODERM CELLS TREATED WITH MESODERMAL INDUCING SUBSTANCES

Using gastrula ectoderm of Cynops pyrrhogaster, the electrophoretic mobility (EPM) of cells was measured. Before EPM measurement, the cells were treated with either LiCl or swimbladder tissue, which are known to possess endo- and mesodermal inducing activity. The EPM of the treated cells increased after 48 hr. A significant difference was observed between the control and experimental series. In addition to the measurements of EPM, histological sections of the explants were examined. Before obvious tissue differentiation occurred in the explants, definitive changes in the EPM values were recorded at 48 and 72 hr after treatment.