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Updated: Jul 27, 2025

Genetic Engineering of Dictyostelium discoideum Cells Based on Selection and Growth on Bacteria
Published on: January 25, 2019
AGGREGATELESS MUTANT DEFECTIVE IN SIGNALING AND RELAYING IN THE CELLULAR SLIME MOLD, DICTYOSTELIUM DISCOIDEUM
1Biological Science, Tsukuba University, Niihari, Ibaraki, 300-31, Japan.
Abstract:
An aggregateless mutant (HT41), isolated from D. discoideum NP14, was examined for the parameters involved in cell aggregation. HT41 cells were induced to express some of these parameters by cyclic AMP pulses. Spontaneous oscillations in light scattering were not observed in the suspension of the mutant cells. A pulse of cyclic AMP caused a monophasic decrease in light scattering, which was similar to the response of pre-aggregation cells of the wild-type. When cyclic AMP pulses were applied on a cell layer, HT41 cells aggregated without forming the streams. These results suggest that HT41 is a mutant defective in signal emission and relay.
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