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Published on: June 9, 2014
Purification and Characterization of Echinonectin, a Carbohydrate-Binding Protein from Sea Urchin Eggs: (sea
Patricia A Veno1, Margaret A Strumski1, William H Kinsey1
1Department of Anatomy and Cell Biology, University of Miami School of Medicine, Miami, Florida 33101, USA.
Abstract:
A galactose-specific carbohydrate binding protein has been identified in eggs and embryos of the sea urchin Lytechinus variegatus. This protein, named echinonectin (Alliegro et al., 1988, J. Cell Biol. 107; 2319-2327) has been described as a cell-substrate adhesion protein functioning during embryonic development. The purified protein has an apparent molecular weight of 220 kDa and exists as a dimer of apparently identical 110 kDa subunits. The carbohytrate specificity of the purified protein was examined through the use of competition assays. The protein has a marked specificity for galactose and fucose and a higher affinity for polymers of galactose or galactose sulfate such as carrageenan.
