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Updated: Jul 27, 2025

Planarian Ovary Dissection for Ultrastructural Analysis and Antibody Staining
Published on: September 10, 2021
CONTENT AND ULTRASTRUCTURAL LOCALIZATION OF TRANSITIONAL METALS IN ASCIDIAN OVARY
Lucio Botte1, Silvia Scippa1, Mario DE Vincentiis1
1II Chair Histology and Embryology, Faculty of Science, Naples University and Zoological Station of Naples. Napoli 80134. Italy.
Abstract:
The content of some transitional metals (Ti, V, Cr. Mn. Fe, Cu) in the ovary of Ascidia malaca and Ciona intestinalis and in the gonads ofPolycarpa gracilis has been reported. The metals were localized histospectrographically and ultrastructurally. In the ovaries of C. intestinalis. the quantitative analysis shows a variable content of metals related to the ovary maturative cycle. The iron is localized in the electron dense cytoplasmic granules ofA. malaca oocytcs and in the cytoplasmic dense formation of P. gracilis test cells. Cl, S, Si and P are present in the test, follicles and inierovarian cells of all examined species. The results are discussed in relation to the zoological position of these species. The presence of metals and Ascidiacee phylogenesis might be correlated.
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