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Updated: Jul 27, 2025
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High Throughput Microinjections of Sea Urchin Zygotes
Published on: January 21, 2014
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LOCALIZATION OF DYNEIN IN SEA URCHIN EGGS DURING CLEAVAGE
H Mohri1, Toshiko Mohri1, I Mabuchi1
1Department of Biology, University of Tokyo, Meguro-ku, Tokyo 153.
Development, Growth & Differentiation
|June 7, 2023
Summary
Dynein, a motor protein, is localized in the mitotic apparatus and egg cortex during sea urchin cell division. Anti-dynein serum inhibited chromosome motion, suggesting a key role for the tubulin-dynein system in mitosis.
Area of Science:
- Cell Biology
- Molecular Biology
- Biochemistry
Background:
- Dynein is a motor protein implicated in various cellular processes.
- Understanding the role of dynein in cell division is crucial for comprehending mitosis.
Purpose of the Study:
- To detect and localize dynein within cleaving sea urchin eggs.
- To investigate the function of dynein during mitosis and cytokinesis.
Main Methods:
- Immunofluorescence microscopy using anti-dynein serum against sea urchin sperm flagella dynein.
- Experiments with isolated mitotic apparatus (MA) and antibody inhibition assays.
Main Results:
- Dynein localized to the mitotic apparatus (asters, spindle) and egg cortex during mitosis.
- Intense staining observed in asters during prophase and the entire MA from metaphase to anaphase.
- Dynein was also present in the egg cortex and cleavage furrow during cytokinesis.
- Anti-dynein serum inhibited chromosome motion in isolated MAs, while anti-myosin serum did not.
Conclusions:
- A tubulin-dynein system plays an active role in mitosis and chromosome movement.
- Dynein is essential for the proper functioning of the mitotic apparatus and cytokinesis.
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