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Updated: Jul 27, 2025
A System for Culturing Iris Pigment Epithelial Cells to Study Lens Regeneration in Newt
Published on: June 22, 2011
In Vivo and in Vitro Experimental Analysis of Lens Regeneration in Larval Xenopus laevis:
Luigi Bosco1, Cristiana Valle1, Daniela Willems1
1Department of Animal and Human Biology, University ≪La Sapienza≫, Via A. Borelli, 50. 00161 Rome, Italy.
Abstract:
After lentectomy of larval Xenopus laevis, the outer cornea undergoes tissue transformation resulting in formation of a new lens. This lens regeneration is triggered and sustained by neural retina. In the present study, lens-forming transformation of the outer cornea was completed in vitro when the outer cornea was cultured within the lentectomized eye-cup. Well-differentiated lens fiber cells, which showed positive immunofluorescence for total crystallins, were also formed when the outer cornea was cultivated with the retina. No lens tissue was formed when the cornea was cultured alone. Lens-forming transformation, originating from the cornea three and five days after lentectomy, completely regressed when the tissue was isolated in vitro. Fom the present and previous findings, we concluded that, the interaction of corneal cells with the retina plays a decisive role in lens regeneration in situ.
