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Updated: Jul 27, 2025

Phosphopeptide Analysis of Rodent Epididymal Spermatozoa
Published on: December 30, 2014
ACROSOME BREAKDOWN IN LEPTODACTYLUS CHAQUENSIS (AMPHIBIA ANURA) SPERMATOZOA
Jorge S Raisman1, Rita W DE Cunio1, Marcelo O Cabada1
1Instituto de Biologia del Desarrollo (INBIODE), Universidad Nacional de Tucumán and Consejo, Nacional de Investigaciones Cientificas y Técnicas (CONICET), Chacabuco 461, San Miguel de Tucumán (4000), R. Argentina.
Abstract:
Acrosome breakdown in Leptodactylus chaquensis is described: during this process acrosome enlarges, becomes round-shaped and finally disrupts. Low tonicity media (0.025 M sucrose and 1/10 Holtfreter's solutions) favor acrosome breakdown and sperm fertility loosing. High tonicity media (0.250 M sucrose and Holtfreter's solutions) maintain acrosomes in an unreacted stage and sperm fertilizing capacity is preserved. Sperm motility does not seem to be a sufficient condition for the sperm to fertilize and also does not seem to be related with acrosome breakdown. The presence of lectins in the incubation media does not modify the time-course of acrosome breakdown.
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