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Updated: Jul 27, 2025
Generation of Aggregates of Mouse Embryonic Stem Cells that Show Symmetry Breaking, Polarization and Emergent Collective Behaviour In Vitro
Published on: November 24, 2015
Internalization of Embryonal Carcinoma Cells when Aggregated with Normal Mouse Embryos:
Kazunori Hanaoka1, Shunzo Kondo2, Michiko Hayasaka1
1Laboratory of Mammalian Developmental Biology, Minami-Ooya, Machida, Tokyo 194, Japan.
Abstract:
In the present study, we examined in detail the process of forming chimeric blastocysts between B242g embryonal carcinoma (EC) cells and normal mouse embryos. Electron microscopic observations of the developing aggregates revealed that the embryonic cells spread over the surface of the EC cells, resulting in the internalization of EC cells in the aggregates. When a single blastomere of an 8-cell embryo was aggregated with EC cells, the blastomere spread over and engulfed the EC cells. These results strongly suggest that EC cells are segregated and become situated in the inside position as the development of an aggregate proceeds, and then they are incorporated into the ICM of a blastocyst.
