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Updated: Jul 27, 2025

Genetic Engineering of Dictyostelium discoideum Cells Based on Selection and Growth on Bacteria
Published on: January 25, 2019
Transcriptional Regulation of Cell-Type-Enriched Genes during Development in Dictyostelium discoideum Analyzed by
Takahiro Morio1, Toshinori Ozaki1, Ikuo Takeuchi1
1Division of Developmental Biology, National Institute for Basic Biology, 38 Nishigonaka, Myodaijicho, Okazaki 444, Japan.
Abstract:
We have previously isolated several cell-type-enriched mRNAs of Dictyostelium discoideum. Since the temporal pattern of appearance and cell-type-enrichment of these RNAs were examined only by determining their accumulation, it was unclear whether their accumulation is regulated at the transcription level or the post-transcriptional level. To distinguish between these two possibilities, we examined the temporal and cell-type-enriched transcription of several of these genes by nuclear run-on assay. The results suggest that some genes are controlled in both temporal accumulation and cell-type-enrichment at the transcriptional level, but post-transcriptional regulation is also important for regulating cell-type enrichment in the case of some other genes.
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