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Efficient Gene Transfer in Chick Retinas for Primary Cell Culture Studies: An Ex-ovo Electroporation Approach
Published on: November 2, 2015
EXPERIMENTAL MANIPULATION OF ALTERNATIVE PATHWAYS OF DIFFERENTIATION IN CULTURES OF EMBRYONIC CHICK NEURAL RETINA
R M Clayton1, D DE Pomerai1, D J Pritchard1
1Institute of Animal Genetics, University of Edinburgh, King's Buildings, West Mains Road, Edinburgh, U. K.
Abstract:
Embryonic chick neural retina cells can transdifferentiate during long-term cell culture into either pigmented epithelium or lens fibres. We have found that some culture conditions influence the choice between these pathways. Pigment cell development is promoted by low initial cell densities and by the use of a medium based on Earle's salt formulation rather than Hank's, while lens fibre development is encouraged by high initial cell densities and by folding the cell sheet into multilayered regions. Some differences in in vitro cell properties of neural retina are reported for two genotypes previously found to exhibit differences in in vitro cell properties of lens epithelial cells.
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