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Updated: Jul 27, 2025
Performing Vaginal Lavage, Crystal Violet Staining, and Vaginal Cytological Evaluation for Mouse Estrous Cycle Staging Identification
Published on: September 15, 2012
A STUDY ON THE ESTRADIOL-INDUCED AUGMENTATION OF A SPECIFIC CELL PRODUCT IN THE VAGINAL EPITHELIUM OF THE NEONATAL
Stein-Ove Døskeland1, Terje Kalland1, John-Gunnar Forsberg1
1The Institute of Anatomy, University of Bergen, 5000 Bergen, Norway.
Abstract:
The epithelial content of an estradiol-sensitive immunological marker (CVA) has been quantified by mixed haemagglutination on tissue sections from the vagina of neonatal mice exposed to different schedules of estradiol treatment. Daily administration of estradiol-17β (5 μg/day) was especially efficient in elevating the CVA content when the hormone was administered during the first four days after birth. Following a single injection of estradiol-17β (5 μg in aqueous suspension) on one of the first five days of life, the vaginal epithelium reacted with a more vigourous CVA accumulation when challenged with estradiol at a later time. The effect was most pronounced for days 2 and 3. The possibility that this early effect of estradiol may involve other mechanisms than those operative in the estradiol action at later stages, is discussed.
