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Updated: Jul 27, 2025
Assay for Neural Induction in the Chick Embryo
Published on: February 13, 2009
INDOLE-3-ACETIC ACID IN CHICK EMBRYOS
Hiroshi Ichimura1, Toshio Yamaki1
1Department of Biology, College of General Education, University of Tokyo, Komaba, Meguroku, Tokyo, 153, Japan.
Abstract:
The occurrence of IAA, a plant-growth-regulating substance of the auxin group, was investigated in developing chick embryos. Paperchromatography followed by the Avena curvature test revealed the presence of a substance with auxin activity and the same Rf as IAA. This substance was identified as IAA by combined gas chromato-graphy-mass spectrometry. The amount of IAA in a unit weight of embryo was almost identical during embryo development, the total amount of IAA in an embryo increasing as it developed. The amount of IAA in the whole egg was also examined and it was found that an egg incubated for 9 days at 37°C contained a larger amount of IAA than one not incubated.
