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Updated: Jul 27, 2025
Trans-inner Cell Mass Injection of Embryonic Stem Cells Leads to Higher Chimerism Rates
Published on: May 29, 2018
Viable Chimeras between Embryonal Carcinoma Cells and Mouse Embryos: Comparison of Aggregation and Injection Methods:
Kazunori Hanaoka1, Michiko Hayasaka1, Takehiko Noguchi2
1Laboratory of Mammalian Developmental Biology, Mitsubishi Kasei Institute of Life Sciences. Machida, Tokyo 194, Japan.
Abstract:
An embryonal carcinoma (EC) cell line having the ability to form chimeric mice was isolated from embryo-derived teratocarcinomas experimentally induced in BALB/cCrSlc mice. This EC cell line, B242 g, was one of the 5 EC cell lines pre-selected based on the ability to incorporate into blastocysts by means of aggregating with 8-cell mouse embryos. Using the B242g EC cells, the effectiveness of producing chimeras was compared between two currently available techniques, aggregation and injection, by examining chimerism of the midgestationally recovered conceptuses and live-born mice. The present result revealed that EC cells studied here were able to form chimeras more efficiently by injection as compared to aggregation method.
