Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Induced Pluripotent Stem Cells01:06

Induced Pluripotent Stem Cells

4.2K
Stem cells are undifferentiated cells that divide and produce different cell types. Ordinarily, cells that have differentiated into a specific cell type are terminally differentiated; however, scientists have found a way to reprogram these mature cells so that they dedifferentiate and return to an unspecialized, proliferative state. These cells are pluripotent like embryonic stem cells—able to produce all cell types—and are called induced pluripotent stem cells (iPSCs).
Somatic...
4.2K
Cancer-Critical Genes I: Proto-oncogenes01:33

Cancer-Critical Genes I: Proto-oncogenes

9.0K
Genes usually encode proteins necessary for the proper functioning of a healthy cell. Mutations can often cause changes to the gene expression pattern, thereby altering the phenotype.
When the function of certain critical genes, especially those involved in cell cycle regulation and cell growth signaling cascades, gets disrupted, it upsets the cell cycle progression. Such cells with unchecked cell cycles start proliferating uncontrollably and eventually develop into tumors.
Such genes that act...
9.0K
Reporter Genes02:11

Reporter Genes

11.6K
Reporter genes are a type of protein-coding gene that are often tagged to a gene of interest. Once inside a target cell, reporter genes usually produce visually identifiable characteristics like fluorescence and luminescence when expressed along with the gene of interest. Thus, reporter genes “report” the presence or absence of genes of interest in an organism, determine the gene expression pattern, or track the physical location of a DNA segment or protein in the cell.
11.6K
Regulation of Expression at Multiple Steps01:23

Regulation of Expression at Multiple Steps

952
The gene expression in cells is regulated at different stages: (i) transcription, (ii) RNA processing, (iii) RNA localization, and (iv) translation. Transcriptional regulation is mediated by regulatory proteins such as transcription factors, activators, or repressors—these control gene expression by initiating or inhibiting the transcription of genes. Once a precursor or pre-mRNA is produced, it undergoes post-transcriptional modification, including 5' capping, splicing, and the...
952
Abnormal Proliferation02:23

Abnormal Proliferation

4.6K
Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the...
4.6K
What is Gene Expression?01:36

What is Gene Expression?

8.6K
A gene is a stretch of DNA that serves as the blueprint for functional RNAs and proteins. Since DNA is comprised  of nucleotides and proteins are comprised of amino acids, a mediator is required to convert the information encoded in DNA into proteins. This mediator is the messenger RNA (mRNA). mRNA copies the blueprint from DNA by a process called transcription. In eukaryotes, transcription occurs in the nucleus by complementary base-pairing with the DNA template. The mRNA is then...
8.6K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Decomposition-based harmonization for quantitative PET imaging across scanners and radiotracers.

Medical physics·2026
Same author

Olfactory Valence-Processing Deficits and Aberrant Brain Network Connectivity Underlie Social Dysfunction in Shank3<sup>-/-</sup> ASD Model Mice.

Neuroscience bulletin·2026
Same author

Brain tau network derived biomarker improves the diagnostic accuracy of Alzheimer's disease.

Brain research bulletin·2026
Same author

Associations Between Early Functional Brain Network Topology and 6-Month Upper Limb Motor Outcomes in Stroke Patients.

Translational stroke research·2026
Same author

Stimulus-Dependent, Not Resting-State, Neural Dysfunction Underlies the Neurobiology of Delayed Ejaculation: A Task-Based fMRI Investigation.

Andrology·2026
Same author

Free water changes and their correlations with multimodal biomarkers in frontotemporal dementia.

Alzheimer's & dementia : the journal of the Alzheimer's Association·2026

Related Experiment Video

Updated: Jul 27, 2025

Overexpressing Long Noncoding RNAs Using Gene-activating CRISPR
13:04

Overexpressing Long Noncoding RNAs Using Gene-activating CRISPR

Published on: March 1, 2019

8.9K

Overexpression of

Jinpiao Zhu1, Chang Chen1, Zhen Li2

  • 1Department of Anesthesiology, Brain Research Center, Department of Neurosurgery, Zhongnan Hospital, Wuhan University, Wuhan, Hubei Province, China.

Neural Regeneration Research
|June 7, 2023
PubMed
Summary
This summary is machine-generated.

Chronic sleep deprivation impairs cognition and brain function. Sirt6 protein levels decrease, but restoring Sirt6 improves cognitive deficits and brain connectivity, offering a potential treatment for sleep disorders.

Keywords:
REM sleepSirt6chronic sleep deprivationcognitive impairmentfunctional connectivityglutamatergic neuronsmetabolic kineticsneuronal-astrocytic glucose metabolismprelimbic cortexsynaptic function

More Related Videos

Co-expression of Multiple Chimeric Fluorescent Fusion Proteins in an Efficient Way in Plants
09:45

Co-expression of Multiple Chimeric Fluorescent Fusion Proteins in an Efficient Way in Plants

Published on: July 1, 2018

9.7K
Overexpression and Purification of Human Cis-prenyltransferase in Escherichia coli
07:35

Overexpression and Purification of Human Cis-prenyltransferase in Escherichia coli

Published on: August 3, 2017

6.7K

Related Experiment Videos

Last Updated: Jul 27, 2025

Overexpressing Long Noncoding RNAs Using Gene-activating CRISPR
13:04

Overexpressing Long Noncoding RNAs Using Gene-activating CRISPR

Published on: March 1, 2019

8.9K
Co-expression of Multiple Chimeric Fluorescent Fusion Proteins in an Efficient Way in Plants
09:45

Co-expression of Multiple Chimeric Fluorescent Fusion Proteins in an Efficient Way in Plants

Published on: July 1, 2018

9.7K
Overexpression and Purification of Human Cis-prenyltransferase in Escherichia coli
07:35

Overexpression and Purification of Human Cis-prenyltransferase in Escherichia coli

Published on: August 3, 2017

6.7K

Area of Science:

  • Neuroscience
  • Metabolic Regulation
  • Sleep Science

Background:

  • Sleep is crucial for energy metabolism, neuronal plasticity, and cognitive function.
  • Sirt6, a NAD+-dependent deacetylase, is a key regulator of energy metabolism impacting transcriptional and enzymatic pathways.
  • Chronic sleep deprivation (CSD) negatively affects brain function and cognition.

Purpose of the Study:

  • To investigate the role of Sirt6 in cerebral function following CSD.
  • To determine if Sirt6 modulation can mitigate CSD-induced cognitive and neural deficits.

Main Methods:

  • C57BL/6J mice were subjected to CSD and viral vector-mediated Sirt6 overexpression in the prelimbic cortex (PrL).
  • Assessed cerebral functional connectivity (FC) via resting-state fMRI, neuronal/astrocyte metabolism using metabolic kinetics ([1-13C] glucose and [2-13C] acetate), dendritic spine density, and synaptic activity (mEPSCs, AP firing rates).
  • Cognitive function was evaluated using comprehensive behavioral tests.

Main Results:

  • CSD led to decreased Sirt6 levels in the PrL, cognitive deficits, and reduced FC between PrL and multiple brain regions.
  • Sirt6 overexpression reversed CSD-induced cognitive impairment and normalized FC.
  • CSD reduced neuronal glucose and GABA synthesis, which Sirt6 overexpression restored.
  • Sirt6 overexpression normalized CSD-induced deficits in action potential firing rates and miniature excitatory postsynaptic currents.

Conclusions:

  • Sirt6 plays a critical role in mitigating cognitive impairment and neural dysfunction caused by CSD.
  • Sirt6 improves cognitive deficits by regulating PrL-associated functional connectivity, neuronal metabolism, and glutamatergic neurotransmission.
  • Sirt6 activation presents a potential therapeutic strategy for sleep disorder-related cognitive impairments.