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Related Experiment Video

Updated: Jul 27, 2025

Sequencing of mRNA from Whole Blood using Nanopore Sequencing

Sequencing of mRNA from Whole Blood using Nanopore Sequencing

Published on: June 3, 2019

High-Throughput Sequencing Reveals N

Furong Wu¹, Shengyu Zhang¹, Chang Fan^2,3

¹Department of Pharmacy, Division of Life Sciences and Medicine, The First Affiliated Hospital of USTC, University of Science and Technology of China, Hefei, Anhui, China.

Current Gene Therapy

|June 7, 2023

Summary

N6-methyladenosine (m6A) modification patterns in long noncoding RNAs (lncRNAs) are linked to liver fibrosis (LF). This study identified specific m6A-modified lncRNAs, revealing their unique methylation patterns and potential roles in LF progression.

Keywords:

N6-methyladenosine high-throughput sequencing liver fibrosis lncRNA meRIP-qPCR regulatory network

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Last Updated: Jul 27, 2025

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Published on: June 3, 2019

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Published on: August 3, 2018

Area of Science:

Molecular Biology
Epigenetics
RNA Biology

Background:

N6-methyladenosine (m6A) is a prevalent RNA modification.
Long noncoding RNAs (lncRNAs) are critical regulatory molecules.
Both m6A and lncRNAs are implicated in liver fibrosis (LF).

Purpose of the Study:

To investigate the role of m6A-modified lncRNAs in liver fibrosis progression.
To identify specific m6A-methylated lncRNAs involved in LF.
To elucidate the m6A methylation patterns of lncRNAs in LF.

Main Methods:

Histopathological examination (HE and Masson staining) of liver tissues.
m6A-modified RNA immunoprecipitation sequencing (m6A-seq) to profile lncRNA methylation.
RNA sequencing (RNA-seq) to assess lncRNA expression levels.
MeRIP-qPCR and RT-qPCR for validation of target lncRNAs.

Main Results:

Identified 415 m6A peaks in 313 lncRNAs in LF tissues, with 98 significant peaks in 84 lncRNAs.
Discovered three lncRNAs (H19, Gm16023, Gm17586) with significant changes in both m6A methylation and expression levels.
Observed distinct alterations in m6A levels (increased for H19 and Gm17586, decreased for Gm16023) and decreased RNA expression for all three.
Constructed a lncRNA-miRNA-mRNA regulatory network to infer functional relationships.

Conclusions:

Demonstrated a unique m6A methylation landscape for lncRNAs in liver fibrosis.
Provided evidence that m6A modification of lncRNAs is associated with the occurrence and development of LF.
Highlighted potential regulatory roles of specific m6A-modified lncRNAs in LF pathogenesis.