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Related Concept Videos

Biofilms01:29

Biofilms

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Biofilms are complex communities of microorganisms encased in a self-produced extracellular polysaccharide matrix attached to surfaces. These microbial consortia can include single or multiple species, providing enhanced survival benefits by forming organized, multilayered structures.The formation of biofilms occurs through four key stages: attachment, colonization, development, and dispersal.During attachment, free-swimming planktonic cells adhere to a surface, often facilitated by...
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The minerals contained in all of the food we consume are essential for our organ systems. However, certain essential minerals, such as calcium, phosphorus, magnesium, manganese, and fluoride, largely affect bone health.
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Calcium is a critical component of bones, especially in the form of calcium phosphate and calcium carbonate. Since the body cannot make calcium, it must be obtained from the diet. However, calcium cannot be absorbed from the small intestine without...
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Disaggregated Nano-Hydroxyapatite (DnHAP) with Inhibitory Effects on Biofilms and Demineralization.

Y Huang1,2,3, Q Han4, X Peng1

  • 1State Key Laboratory of Oral Diseases, West China Hospital of Stomatology, National Clinical Research Centre for Oral Diseases, Sichuan University, Chengdu, China.

Journal of Dental Research
|June 7, 2023
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Disaggregated nano-hydroxyapatite (DnHAP) effectively inhibits the growth and acid production of dental biofilms. This study shows DnHAP reduces demineralization, offering a promising strategy against dental caries.

Keywords:
Candida albicansStreptococcus mutansbiofilm regrowthdeagglomerationdental cariesoral microbiome

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Area of Science:

  • Biomaterials Science
  • Microbiology
  • Dental Research

Background:

  • Nano-hydroxyapatite (nHAP) is known for biocompatibility and remineralization.
  • The antibacterial and anti-biofilm effects of nHAP, particularly disaggregated nHAP (DnHAP), require further clarification.

Purpose of the Study:

  • To investigate the inhibitory effects of DnHAP on established biofilms.
  • To assess DnHAP's impact on dental demineralization and virulence factors.

Main Methods:

  • In vitro models of single-species, dual-species, and saliva-derived microcosm biofilms were used.
  • Biofilms were treated repeatedly with DnHAP.
  • Assays included viability, lactic acid production, biomass, demineralization (transverse microradiography), and 16S rRNA gene sequencing.

Main Results:

  • DnHAP significantly inhibited metabolism, lactic acid, and biomass in single/dual-species biofilms.
  • Saliva-derived biofilms treated with DnHAP showed reduced metabolic activity and lactic acid production.
  • DnHAP significantly reduced enamel demineralization, lesion depth, and volume.
  • Biofilm microbial diversity remained unchanged after DnHAP treatment.

Conclusions:

  • DnHAP demonstrates significant inhibitory effects against regrown dental biofilms.
  • DnHAP effectively reduces enamel demineralization, suggesting potential for caries management.
  • DnHAP presents a promising therapeutic agent for controlling dental caries progression.