Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Disassembly of Intermediate Filaments01:35

Disassembly of Intermediate Filaments

2.1K
Intermediate filaments (IFs) do not undergo spontaneous disassembly. Enzymes, kinases, and phosphatases add and remove phosphates from specific sites to regulate their disassembly. The IF concentration in the cytoplasm also regulates the disassembly. If the concentration crosses a threshold, it activates the protein kinases in the vicinity, allowing the phosphorylation of IFs.
Keratin proteins, found at the cell periphery near cell junctions, undergo a cycle of assembly and disassembly. In Type...
2.1K
Formation of Intermediate Filaments00:57

Formation of Intermediate Filaments

3.1K
Intermediate filaments are cytoskeletal proteins with higher tensile strength and flexibility than microfilaments and microtubules. Unlike the other two cytoskeletal proteins, intermediate filament formation lacks the enzymatic activity to hydrolyze nucleotides like ATP and GTP to generate energy for polymerization. Therefore, the formation of intermediate filaments is multistep self-assembly. The involvement of any accessory proteins in intermediate filament formation has not yet been...
3.1K
Types of Intermediate Filaments01:31

Types of Intermediate Filaments

3.7K
The intermediate filaments are an essential component of the cytoskeleton. Presently six types of intermediate filament have been identified. Type I and II are acidic and basic keratin proteins. Type III is of mesodermal origin and comprises four proteins: vimentin, desmin, glial fibrillary acidic protein (GFAP), and peripherin. Vimentin is commonly found in mesenchymal cells, desmin in muscle cells, GFAP in astrocytes, while peripherin is found in peripheral nervous system neurons (PNS). Type...
3.7K
The Structure of Intermediate Filaments01:19

The Structure of Intermediate Filaments

4.1K
The intermediate filaments are one of three widely studied cytoskeletal filaments. They are so named as their diameter (10 nm) is in between that of microfilaments (7 nm) and the microtubules (25 nm).  These filaments are highly stable and can remain intact when exposed to high salt concentrations and detergents. These filaments are responsible for providing stability and mechanical support to the cells. They also help in cell adhesion and maintaining tissue integrity.
Intermediate...
4.1K
Adaptability of Cytoskeletal Filaments01:12

Adaptability of Cytoskeletal Filaments

3.8K
The cytoskeleton is a complex dynamic structure performing varied functions based on cellular requirements. The adaptability of the individual filaments in the cytoskeleton determines their ability to perform various functions within the cell. It can undergo rapid reorganization during processes like cell division or remain stable for several hours as in the interphase. The adaptability of these filaments depends on stringent regulatory mechanisms. The microfilament and microtubules of the...
3.8K
Destabilization of Microtubules01:45

Destabilization of Microtubules

2.8K
The destabilization of microtubules can occur during different stages of the microtubule lifecycle, such as nucleation or elongation. It can take place at either end of the microtubule or in the microtubule lattices as a whole. The lifespan of individual microtubules within a cell varies according to the cell type and stage of the cell cycle. During interphase, the lifespan of the microtubule is about 30 minutes, while during cell division, it is about 15 minutes. In axonal microtubules of...
2.8K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Urine peptides enable non-invasive differential diagnosis of nodular diabetic nephropathy.

Diabetes research and clinical practice·2026
Same author

The role of platelet-derived growth factor in uremic vascular calcification.

Kidney international·2026
Same author

LGL-1 and the RhoGAP protein PAC-1 redundantly polarize the Caenorhabditis elegans embryonic epidermis.

The Journal of cell biology·2026
Same author

LGL-1 and the RhoGAP protein PAC-1 redundantly polarize the <i>C. elegans</i> embryonic epidermis.

bioRxiv : the preprint server for biology·2026
Same author

Impact of amino acid supplementation on cardiovascular and chronic kidney diseases: a systematic review.

Cardiovascular research·2026
Same author

The impact of gut-liver-derived mediators on the organ crosstalk with brain, heart, and kidney: A systematic review.

Molecular metabolism·2025
Same journal

Non-canonical amino acid incorporation enables minimally disruptive labeling of stress granule and TDP-43 proteinopathy.

eLife·2026
Same journal

Analysis of dendritic input currents during place field dynamics.

eLife·2026
Same journal

TopoMetry systematically learns and evaluates the latent geometry of single-cell data.

eLife·2026
Same journal

Navigating the path: Advice to physician-scientists on choosing a clinical specialty.

eLife·2026
Same journal

Neural activity profiles reveal overlapping, intermingled subpopulations spanning area borders in mouse sensorimotor cortex.

eLife·2026
Same journal

The exquisite mechanics of a tsetse bite.

eLife·2026
See all related articles

Related Experiment Video

Updated: Jul 27, 2025

Using Microfluidics and Fluorescence Microscopy to Study the Assembly Dynamics of Single Actin Filaments and Bundles
08:02

Using Microfluidics and Fluorescence Microscopy to Study the Assembly Dynamics of Single Actin Filaments and Bundles

Published on: May 5, 2022

2.7K

Intermediate filament network perturbation in the

Florian Geisler1, Sanne Remmelzwaal2, Vera Jankowski3

  • 1Institute of Molecular and Cellular Anatomy, RWTH Aachen University, Aachen, Germany.

Elife
|June 7, 2023
PubMed
Summary
This summary is machine-generated.

Intermediate filaments (IFs) organize cell structure. Suppressing aberrant IF networks in Caenorhabditis elegans mutants corrects structural and functional defects, revealing IF network importance in cell and tissue function.

Keywords:
C. eleganscell biologyendotubeintermediate filamentintestine

More Related Videos

Imaging Intermediate Filaments and Microtubules with 2-dimensional Direct Stochastic Optical Reconstruction Microscopy
14:23

Imaging Intermediate Filaments and Microtubules with 2-dimensional Direct Stochastic Optical Reconstruction Microscopy

Published on: March 6, 2018

10.9K
Isolation of Intermediate Filament Proteins from Multiple Mouse Tissues to Study Aging-associated Post-translational Modifications
09:29

Isolation of Intermediate Filament Proteins from Multiple Mouse Tissues to Study Aging-associated Post-translational Modifications

Published on: May 18, 2017

8.5K

Related Experiment Videos

Last Updated: Jul 27, 2025

Using Microfluidics and Fluorescence Microscopy to Study the Assembly Dynamics of Single Actin Filaments and Bundles
08:02

Using Microfluidics and Fluorescence Microscopy to Study the Assembly Dynamics of Single Actin Filaments and Bundles

Published on: May 5, 2022

2.7K
Imaging Intermediate Filaments and Microtubules with 2-dimensional Direct Stochastic Optical Reconstruction Microscopy
14:23

Imaging Intermediate Filaments and Microtubules with 2-dimensional Direct Stochastic Optical Reconstruction Microscopy

Published on: March 6, 2018

10.9K
Isolation of Intermediate Filament Proteins from Multiple Mouse Tissues to Study Aging-associated Post-translational Modifications
09:29

Isolation of Intermediate Filament Proteins from Multiple Mouse Tissues to Study Aging-associated Post-translational Modifications

Published on: May 18, 2017

8.5K

Area of Science:

  • Cell Biology
  • Cytoskeleton Dynamics
  • Molecular Genetics

Background:

  • Intermediate filaments (IFs) are crucial cytoskeletal components in metazoans.
  • A key debate questions whether IF network organization dictates or merely reflects cellular function.
  • Mutants in mitogen-activated protein kinase (MAPK) SMA-5 disrupt intestinal IF organization, causing structural and functional defects.

Purpose of the Study:

  • To investigate the role of IF network organization in cell and tissue function.
  • To identify suppressors of structural and functional deficiencies caused by aberrant IF networks.
  • To elucidate the mechanism by which IF network organization is regulated.

Main Methods:

  • Utilizing Caenorhabditis elegans as a model organism.
  • Generating and analyzing mutants affecting IF network organization (sma-5 mutants).
  • Assessing the effects of IF polypeptide IFB-2 on structural and functional phenotypes.

Main Results:

  • The IF polypeptide IFB-2 efficiently suppresses structural and functional defects in sma-5 mutants by resolving aberrant IF networks.
  • Perturbed IF network formation is linked to hyperphosphorylation of IFB-2.
  • IFB-2's rescuing activity is IF isotype-specific and effective in other mutants affecting cytoskeletal linkers (IFO-1) and IF-associated proteins (BBLN-1).

Conclusions:

  • Deranged IF networks have detrimental consequences for cell and tissue function.
  • IF network organization plays a critical role in maintaining cellular and organismal health.
  • These findings have implications for diseases associated with altered IF network organization.