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Role of the

Rodrigo Arias-Cartin1, Katayoun Kazemzadeh Ferizhendi2, Emmanuel Séchet1

  • 1Département de Microbiologie, Institut Pasteur, Université Paris-Cité, UMR CNRS 6047, SAMe Unit , Paris, France.

Mbio
|June 7, 2023
PubMed
Summary
This summary is machine-generated.

Escherichia coli synthesizes ubiquinone (UQ) without oxygen using UbiUV enzymes, crucial for anaerobic respiration and uracil production. This anaerobic UQ synthesis allows efficient adaptation to changing oxygen levels.

Keywords:
E. coliFnrUbiTUVquinonerespiration

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Area of Science:

  • Microbiology
  • Molecular Biology
  • Biochemistry

Background:

  • Isoprenoid quinones like ubiquinone (UQ) and demethylmenaquinones (DMK) are vital for cellular respiration.
  • UQ is typically associated with aerobic conditions, while DMK is used anaerobically in E. coli and related bacteria.

Purpose of the Study:

  • To characterize the regulation of the anaerobic UQ biosynthesis genes ubiTUV in E. coli.
  • To investigate the physiological roles of anaerobic UQ synthesis.
  • To elucidate the mechanism of O2-independent UQ hydroxylation.

Main Methods:

  • Gene transcription analysis of ubiTUV operons.
  • Phenotypic analysis of menA mutant strains.
  • 18O2 labeling experiments.
  • Genetic studies.

Main Results:

  • The ubiTUV genes are transcribed as two divergent operons regulated by the O2-sensing Fnr regulator.
  • Anaerobic UQ synthesis via UbiUV is essential for nitrate respiration and uracil biosynthesis.
  • UbiUV enzymes perform O2-independent hydroxylation of UQ precursors.
  • ubiT plays a critical role in the transition from anaerobic to aerobic conditions.

Conclusions:

  • E. coli possesses a novel anaerobic UQ biosynthesis pathway essential for adapting to fluctuating oxygen environments.
  • Anaerobic UQ supports key metabolic processes including respiration and pyrimidine synthesis.
  • This pathway is crucial for bacterial colonization in the gut and has implications for understanding facultative anaerobic pathogens.