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Updated: Jul 27, 2025

Collecting Saliva and Measuring Salivary Cortisol and Alpha-amylase in Frail Community Residing Older Adults via Family Caregivers
Published on: December 18, 2013
Salivary cortisol as a marker for assessing the problem-focused coping style of stressed students during the first
Mitsuo Nagane1, Yoshinori Oyama2, Fuminobu Tamalu1
1Department of Physiology Saitama Medical University Moroyama-machi Saitama Japan.
Background And Aims:
First-year students encounter substantial stress when they enter university. Their mental health often depends upon how well they cope with the stress of university life. Salivary components are well known to reflect the stress status of the students; however, the relationship between salivary components and coping styles remains unknown.
Methods:
In this study, 54 healthy first-year students voluntarily completed a questionnaire that addressed three different coping styles: problem-focused, emotion-focused, and escape-focused. We simultaneously collected salivary samples from students in the classroom and measured concentrations of salivary cortisol and α-amylase by enzyme-linked immunosorbent assays over 4 months.
Results:
We examined the relationship between coping style and salivary cortisol concentrations and found that the mean salivary cortisol concentrations were significantly lower in students who had a higher Likert-type score for the problem-focused coping style than in students who had a lower score. The difference in the mean cortisol concentrations between the two groups increased over time. However, we observed no apparent correlation between α-amylase concentrations and Likert scores of the three coping styles.
Conclusion:
These results suggest that salivary cortisol concentrations might reflect the stress-coping status, particularly involving the problem-focused coping style.
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