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Related Concept Videos

The Extracellular Matrix01:42

The Extracellular Matrix

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Related Experiment Video

Updated: Jul 27, 2025

Vasodilation of Isolated Vessels and the Isolation of the Extracellular Matrix of Tight-skin Mice
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Pipeline for precise insoluble matrisome coverage in tissue extracellular matrices.

Wei Chen1,2, Wen Zhang3,4, Ning Zhang3,4

  • 1Beijing Clinical Research Institute, Beijing, China.

Frontiers in Bioengineering and Biotechnology
|June 7, 2023
PubMed
Summary

Researchers developed a new method to analyze insoluble extracellular matrix (ECM) proteins in tissues. This technique improves understanding of ECM

Keywords:
LC-MS/MSdecellularizationmatrisomeproteomicsodium dodecyl sulfate

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Enrichment of Extracellular Matrix Proteins from Tissues and Digestion into Peptides for Mass Spectrometry Analysis
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Area of Science:

  • Biochemistry and Molecular Biology
  • Proteomics
  • Tissue Engineering

Background:

  • The extracellular matrix (ECM) is crucial for tissue structure and function, regulating cell behavior through mechanical and biochemical signals.
  • Dysregulation of ECM composition is implicated in diseases like fibrosis and cancer, but analyzing insoluble ECM proteins remains challenging.
  • Current methods for comprehensive insoluble matrisome analysis are limited, hindering a full understanding of ECM's role in health and disease.

Purpose of the Study:

  • To develop and validate an enhanced workflow for thorough tissue decellularization and analysis of insoluble ECM proteins.
  • To establish a reliable pipeline for accurate identification and quantification of highly insoluble matrisome proteins.
  • To provide a cost-effective and efficient method for exploring the insoluble matrisome in various tissues.

Main Methods:

  • An enhanced sodium dodecyl sulfonate (E-SDS) workflow was implemented for comprehensive tissue decellularization.
  • A pipeline was established for the accurate identification and quantification of insoluble extracellular matrix (ECM) matrisome proteins.
  • Mass spectrometry (MS) analysis and experimental validations were used to assess the purity of decellularized ECM (dECM) scaffolds.

Main Results:

  • The developed pipeline successfully analyzed the insoluble matrisome landscape across nine mouse organs.
  • Decellularized ECM (dECM) scaffolds showed minimal contamination from cellular debris, confirmed by mass spectrometry (MS) and validation experiments.
  • The method provides a thorough coverage of highly insoluble ECM matrisome proteins.

Conclusions:

  • The proposed enhanced sodium dodecyl sulfonate (E-SDS) workflow and subsequent pipeline offer a robust method for insoluble matrisome analysis.
  • This approach overcomes previous methodological bottlenecks in studying insoluble ECM components.
  • The pipeline is a valuable, low-cost, and effective tool for proteomic studies aiming to advance ECM discovery and understand its role in pathophysiology.