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Fabricating High-viscosity Droplets using Microfluidic Capillary Device with Phase-inversion Co-flow Structure
Published on: April 17, 2018
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Dynamic catcher for stabilization of high-viscosity extrusion jets
R Bruce Doak1, Robert L Shoeman1, Alexander Gorel1
1Department of Biomolecular Mechanisms, Max Planck Institute for Medical Research, Jahnstrasse 29, 69120 Heidelberg, Germany.
Journal of Applied Crystallography
|June 7, 2023
Summary
A new revolving cylindrical collector, or
Area of Science:
- Crystallography
- Materials Science
- Biophysics
Background:
- Serial X-ray crystallography requires stable delivery of microcrystals.
- High-viscosity extrusion injection methods often suffer from free-jet instabilities.
- Existing methods for microcrystal delivery can be complex or costly.
Purpose of the Study:
- To develop a simple and inexpensive device for microcrystal delivery.
- To mitigate instabilities in high-viscosity extrusion injection.
- To facilitate microcrystal delivery for serial X-ray crystallography.
Main Methods:
- Design and implementation of a revolving cylindrical collector ('catcher').
- Utilizing the collector to stabilize the free jet during extrusion injection.
- Integrating the device with serial diffraction X-ray crystallography workflows.
Main Results:
- The revolving collector effectively reduces free-jet instabilities.
- Stable microcrystal delivery was achieved from high-viscosity solutions.
- The device is simple to construct and inexpensive to implement.
Conclusions:
- The described catcher is a practical solution for microcrystal delivery challenges.
- This innovation enhances the feasibility of serial X-ray crystallography for viscous samples.
- The device offers a cost-effective method for improving diffraction data quality.
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