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Serum selenium assay following serum ferritin assay
American Journal of Epidemiology
|August 1, 1986
Summary
Testing serum ferritin levels did not affect selenium measurements. This study found no significant difference in selenium values whether or not serum ferritin was previously measured.
Area of Science:
- Biochemistry
- Analytical Chemistry
- Clinical Chemistry
Background:
- Ferritin is an iron-storage protein.
- Selenium is an essential trace element.
- Accurate measurement of serum selenium is important for nutritional and clinical assessments.
Purpose of the Study:
- To investigate whether prior radioimmunoassay for ferritin impacts subsequent neutron activation assay for selenium in human serum.
- To determine if ferritin testing affects selenium concentration measurements.
Main Methods:
- Serum samples from 10 individuals were prepared.
- Samples were divided into six subsamples each.
- Three subsamples underwent radioimmunoassay for ferritin.
- All six subsamples were analyzed for selenium using neutron activation assay.
Main Results:
- No statistically significant difference was observed in selenium values between serum samples that were tested for ferritin and those that were not.
- The process of ferritin testing did not alter the measured selenium concentrations.
Conclusions:
- Radioimmunoassay for ferritin does not interfere with the neutron activation assay measurement of selenium in human serum.
- This finding supports the reliability of sequential testing for both ferritin and selenium in clinical diagnostics.
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