Transcriptome analysis of the gene expression of M

Area of Science:

• Animal Science
• Nutritional Biochemistry
• Molecular Biology

Background:

• Methionine (Met) is a crucial amino acid for skeletal muscle development.
• Understanding Met's role is vital for optimizing broiler chicken growth and meat quality.

Purpose of the Study:

• To investigate the effects of dietary methionine restriction on gene expression in the M. iliotibialis lateralis muscle of broiler chicks.
• To identify specific genes and pathways affected by methionine restriction.

Main Methods:

• Broiler chicks were fed either a control or a methionine-restricted diet for 63 days.
• Growth performance and M. iliotibialis lateralis development were assessed.
• Transcriptome analysis was performed on muscle samples to identify differentially expressed genes (DEGs).

Main Results:

• Methionine restriction did not affect overall broiler growth performance.
• Muscle development in the M. iliotibialis lateralis was hindered by methionine restriction.
• Transcriptome analysis identified 247 upregulated and 173 downregulated DEGs, enriched in 10 pathways.
• Genes such as CSRP3, KY, FHL1, LMCD1, and MYOZ2 were downregulated.

Conclusions:

• Dietary methionine restriction negatively impacts M. iliotibialis lateralis development in broiler chicks.
• Specific genes including CSRP3, KY, FHL1, LMCD1, and MYOZ2 are potentially involved in this developmental process.