Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Genomics02:02

Genomics

36.5K
Genomics is the science of genomes: it is the study of all the genetic material of an organism. In humans, the genome consists of information carried in 23 pairs of chromosomes in the nucleus, as well as mitochondrial DNA. In genomics, both coding and non-coding DNA is sequenced and analyzed. Genomics allows a better understanding of all living things, their evolution, and their diversity. It has a myriad of uses: for example, to build phylogenetic trees, to improve productivity and...
36.5K
Sanger Sequencing01:57

Sanger Sequencing

755.2K
DNA sequencing is a fundamental technique that is routinely used in the biological sciences. This method can be applied to a range of questions at different scales - from the sequencing of a cloned DNA fragment or the study of a mutation in a gene up to whole-genome sequencing. However, despite the widespread use of sequencing today, it was not until 1977 that Fredrick Sanger and his collaborators developed the chain-termination method to decode DNA sequences. It relies on the separation of a...
755.2K
Genome Annotation and Assembly03:36

Genome Annotation and Assembly

18.9K
The genome refers to all of the genetic material in an organism. It can range from a few million base pairs in microbial cells to several billion base pairs in many eukaryotic organisms. Genome assembly refers to the process of taking the DNA sequencing data and putting it all back together in a correct order to create a close representation of the original genome. This is followed by the identification of functional elements on the newly assembled genome, a process called genome annotation.
18.9K
Next-generation Sequencing03:00

Next-generation Sequencing

91.7K
The first human genome sequencing project cost $2.7 billion and was declared complete in 2003, after 15 years of international cooperation and collaboration between several research teams and funding agencies. Today, with the advent of next-generation sequencing technologies, the cost and time of sequencing a human genome have dropped over 100 fold.
Next-Generation Sequencing Methods
Although all next-generation methods use different technologies, they all share a set of standard features....
91.7K
Maxam-Gilbert Sequencing01:05

Maxam-Gilbert Sequencing

11.3K
In the same year as the discovery of the Sanger sequencing method, another group of scientists, Allan Maxam and Walter Gilbert, demonstrated their chemical-cleavage method for DNA sequencing. The Maxam-Gilbert method relies on using different chemicals that can cleave the DNA sequence at specific sites, the separation of resulting DNA fragments of variable size using electrophoresis, and deciphering the DNA sequence from the resulting gel bands.
Challenges of the Maxam-Gilbert Method
The...
11.3K
RNA-seq03:21

RNA-seq

10.1K
RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
10.1K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Transcriptomic and metabolomic profiling of <i>Polygonatum cyrtonema</i> Hua across different growth years.

Frontiers in plant science·2026
Same author

Steering Diels-Alder Reaction with Mechanical Force: Structure Deformation versus Electron Rearrangement.

The journal of physical chemistry. A·2026
Same author

Computational Insights into the Mechanism of [4 + 2] Cycloaddition of Aza-ortho-quinone Methides with Terminal Alkynes.

The journal of physical chemistry. A·2026
Same author

LURP1, a SARD1-regulated protein, coordinates with the helper NLR ADR1 to promote plant immunity.

The New phytologist·2026
Same author

Cu(I)-Catalyzed (4+1) Tandem Annulation of Terminal Alkynes with 2-(Tosylmethyl)phenols: Access to 2,3-Disubstituted Benzofurans and Mechanism Insights.

The Journal of organic chemistry·2026
Same author

Profiles of Invalidating Family Environments and Their Associations With Borderline Personality Features, Depression, and Anxiety in Children and Adolescents.

Personality and mental health·2026

Related Experiment Video

Updated: Jul 27, 2025

Ultra-long Read Sequencing for Whole Genomic DNA Analysis
10:34

Ultra-long Read Sequencing for Whole Genomic DNA Analysis

Published on: March 15, 2019

22.9K

Complete Genome Sequence of "

Yalu Li1, Xiao-Hua Yan2, Yanzhi Liu1

  • 1Shanghai Center for Plant Stress Biology, CAS Center for Excellence in Molecular Plant Sciences, Chinese Academy of Sciences, Shanghai, China.

Microbiology Resource Announcements
|June 7, 2023
PubMed
Summary

Researchers present the complete genome sequence of "Candidatus Phytoplasma aurantifolia" TB2022. This phytoplasma is linked to sweet potato little leaf disease in China.

More Related Videos

Hybrid De Novo Genome Assembly for the Generation of Complete Genomes of Urinary Bacteria using Short- and Long-read Sequencing Technologies
12:08

Hybrid De Novo Genome Assembly for the Generation of Complete Genomes of Urinary Bacteria using Short- and Long-read Sequencing Technologies

Published on: August 20, 2021

5.1K
Novel Sequence Discovery by Subtractive Genomics
09:40

Novel Sequence Discovery by Subtractive Genomics

Published on: January 25, 2019

8.7K

Related Experiment Videos

Last Updated: Jul 27, 2025

Ultra-long Read Sequencing for Whole Genomic DNA Analysis
10:34

Ultra-long Read Sequencing for Whole Genomic DNA Analysis

Published on: March 15, 2019

22.9K
Hybrid De Novo Genome Assembly for the Generation of Complete Genomes of Urinary Bacteria using Short- and Long-read Sequencing Technologies
12:08

Hybrid De Novo Genome Assembly for the Generation of Complete Genomes of Urinary Bacteria using Short- and Long-read Sequencing Technologies

Published on: August 20, 2021

5.1K
Novel Sequence Discovery by Subtractive Genomics
09:40

Novel Sequence Discovery by Subtractive Genomics

Published on: January 25, 2019

8.7K

Area of Science:

  • Plant pathology
  • Bacteriology
  • Genomics

Background:

  • "Candidatus Phytoplasma aurantifolia" is an economically significant plant pathogen.
  • Sweet potato little leaf disease causes substantial crop losses in affected regions.
  • Phytoplasma genomes are essential for understanding their biology and developing control strategies.

Purpose of the Study:

  • To present the complete genome sequence of "Candidatus Phytoplasma aurantifolia" TB2022.
  • To provide genomic resources for studying the pathogen's virulence and host interactions.
  • To aid in the development of diagnostic and management strategies for sweet potato little leaf disease.

Main Methods:

  • Whole-genome sequencing of "Candidatus Phytoplasma aurantifolia" TB2022.
  • Bioinformatic analysis of the generated genome sequence.
  • Assembly of a 670,073-bp circular chromosome.

Main Results:

  • The complete genome sequence of "Candidatus Phytoplasma aurantifolia" TB2022 was successfully assembled.
  • The genome comprises a single circular chromosome of 670,073 base pairs.
  • This genomic data provides a foundation for further research into the pathogen.

Conclusions:

  • The availability of the complete genome sequence facilitates in-depth analysis of "Candidatus Phytoplasma aurantifolia".
  • This resource will advance understanding of phytoplasma-plant interactions and disease mechanisms.
  • Genomic insights are crucial for effective disease management in sweet potato cultivation.