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Biosensor-based High Throughput Biopanning and Bioinformatics Analysis Strategy for the Global Validation of Drug-protein Interactions
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Biosensor Assays Types and Their Roles Toward Ligand-Receptor Interactions in Drug Discovery
Garima Gupta1, Kanupriya Jha1, Sarika Chaudhary1
1Department of Biotechnology, School of Engineering and Applied Sciences, Bennett University, Greater Noida, India.
Assay and Drug Development Technologies
|June 7, 2023
Summary
This review explores biosensors for drug-target interactions, highlighting label-free systems and advanced 3D models. These innovations improve drug discovery efficiency and reduce costs, paving the way for better therapeutics.
Area of Science:
- Biomedical Engineering
- Pharmacology
- Biosensor Technology
Background:
- Ligand-receptor interactions (LRIs) are fundamental to cellular processes and have been leveraged for sensitive biosensor development.
- Drug-target interactions, a key LRI, are crucial for understanding biological processes and developing novel therapeutics.
- Label-free biosensor systems offer advantages over traditional methods by avoiding labeling-associated issues, saving time and effort.
Purpose of the Study:
- To provide an in-depth analysis of biosensor assays based on drug-target interactions.
- To evaluate the advantages and limitations of various biosensor types regarding cost, sensitivity, and selectivity.
- To discuss the industrial applications of these biosensor technologies in drug discovery and development.
Main Methods:
- Review of existing literature on biosensor assays for drug-target interactions.
- Analysis of label-free biosensing approaches and their benefits.
- Evaluation of advanced culture models (3D, organoids, organ-on-chip) in drug screening.
- Discussion of the impact of multiplexing and nanotechnology on biosensor efficacy.
Main Results:
- Label-free biosensors circumvent issues like conformational changes and off-site labeling, streamlining assay development.
- Three-dimensional (3D) culture models offer more physiologically relevant in vitro approaches compared to traditional 2D models.
- Advancements in multiplexing and nanotechnology are enhancing biosensor efficacy, leading to miniaturized and point-of-care applications.
Conclusions:
- Biosensors based on drug-target interactions are vital tools for drug discovery and development.
- Label-free and advanced 3D culture technologies represent significant progress in improving the efficiency and predictivity of drug screening.
- Continued innovation in biosensor technology, including nanotechnology, promises further advancements in diagnostics and therapeutics.
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