Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Scanning Electron Microscopy01:07

Scanning Electron Microscopy

4.3K
A scanning electron microscope (SEM) is used to study the surface features of a sample by using an electron beam that scans the sample surface in a two-dimensional manner. Typically, areas between ~1 centimeter to 5 micrometers in width can be imaged. SEM can be used to image bacteria, viruses, tissues as well as larger samples like insects. Conventional SEM gives a magnification ranging from 20X to 30,000X and spatial resolution of 50 to 100 nanometers.
Fundamental Principles
Accelerated...
4.3K
Confocal Fluorescence Microscopy01:16

Confocal Fluorescence Microscopy

13.4K
Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
13.4K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Voice disturbances in Parkinson's disease and essential tremor: A cross-sectional acoustic comparison.

Parkinsonism & related disorders·2026
Same author

Is thalamic deep brain stimulation the right target to improve laryngeal dystonia symptoms?

Dystonia (Lausanne, Switzerland)·2026
Same author

Large language model analysis of real-world phone calls reveals prodromal and progressive biomarker of parkinsonism: A two-year proof-of-concept study.

PLOS digital health·2026
Same author

Speech Biomarkers for Quantifying Effects of Subthalamic Deep Brain Stimulation in Parkinson's Disease.

Annals of neurology·2026
Same author

High-Performance Infrared Photodetectors Based on Graphene Nanoribbon Vertical Heterojunctions via Dissociated Double-Walled Carbon Nanotubes.

Nanomaterials (Basel, Switzerland)·2026
Same author

Remote smartphone-based spoken language screening predicts clinical markers in Huntington's disease.

Journal of neural transmission (Vienna, Austria : 1996)·2026

Related Experiment Video

Updated: Jul 27, 2025

Highly Resolved Intravital Striped-illumination Microscopy of Germinal Centers
10:07

Highly Resolved Intravital Striped-illumination Microscopy of Germinal Centers

Published on: April 9, 2014

10.1K

Single scan STEM-EMCD in 3-beam orientation using a quadruple aperture.

Hasan Ali1, Sharath Kumar Manjeshwar Sathyanath2, Cheuk-Wai Tai3

  • 1Department of Materials and Environmental Chemistry, Stockholm University, 106 91 Stockholm, Sweden; Department of Materials Science and Engineering, Uppsala University, Box 534, 75121, Uppsala, Sweden.

Ultramicroscopy
|June 7, 2023
PubMed
Summary

Acquiring multiple electron energy loss spectra (EELS) for electron magnetic circular dichroism (EMCD) is challenging. This study introduces a quadruple aperture to collect all necessary EELS data in a single scan, simplifying EMCD analysis.

More Related Videos

Quantitative Atomic-Site Analysis of Functional Dopants/Point Defects in Crystalline Materials by Electron-Channeling-Enhanced Microanalysis
07:24

Quantitative Atomic-Site Analysis of Functional Dopants/Point Defects in Crystalline Materials by Electron-Channeling-Enhanced Microanalysis

Published on: May 10, 2021

6.2K
Preparation and Observation of Thick Biological Samples by Scanning Transmission Electron Tomography
08:04

Preparation and Observation of Thick Biological Samples by Scanning Transmission Electron Tomography

Published on: March 12, 2017

9.4K

Related Experiment Videos

Last Updated: Jul 27, 2025

Highly Resolved Intravital Striped-illumination Microscopy of Germinal Centers
10:07

Highly Resolved Intravital Striped-illumination Microscopy of Germinal Centers

Published on: April 9, 2014

10.1K
Quantitative Atomic-Site Analysis of Functional Dopants/Point Defects in Crystalline Materials by Electron-Channeling-Enhanced Microanalysis
07:24

Quantitative Atomic-Site Analysis of Functional Dopants/Point Defects in Crystalline Materials by Electron-Channeling-Enhanced Microanalysis

Published on: May 10, 2021

6.2K
Preparation and Observation of Thick Biological Samples by Scanning Transmission Electron Tomography
08:04

Preparation and Observation of Thick Biological Samples by Scanning Transmission Electron Tomography

Published on: March 12, 2017

9.4K

Area of Science:

  • Materials Science
  • Condensed Matter Physics
  • Electron Microscopy

Background:

  • Electron Magnetic Circular Dichroism (EMCD) requires multiple angle-resolved electron energy loss spectra (EELS).
  • Acquiring these spectra involves scanning the same sample area multiple times, which is prone to errors from beam damage, contamination, and drift.
  • Precise spatial registration between scans is critical for accurate magnetic information.

Purpose of the Study:

  • To develop a novel method for acquiring EELS data for EMCD in a single electron beam scan.
  • To overcome the challenges associated with multiple scans in EMCD experiments.
  • To enable more precise and reliable local magnetic measurements.

Main Methods:

  • Utilized a custom-made quadruple aperture to collect four EELS spectra simultaneously.
  • Performed EMCD analysis using data acquired in a single scan.
  • Investigated quantitative EMCD results with sub-nanometer probe sizes and compared different detector geometries.

Main Results:

  • Successfully acquired all necessary EELS spectra for EMCD analysis in a single electron beam scan.
  • Eliminated complexities related to spatial registration and beam-induced sample modifications.
  • Demonstrated quantitative EMCD results for a sub-nanometer probe size.

Conclusions:

  • The custom quadruple aperture significantly simplifies EMCD experiments.
  • This single-scan approach enhances the accuracy and reliability of local magnetic information.
  • The method is applicable for advanced electron microscopy techniques requiring precise spectral acquisition.