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Lysosomal Hydrolases

Lysosomal Hydrolases

Lysosomes are the site for the degradation of macromolecules and biological polymers released during membrane trafficking events such as secretory, endocytic, autophagic, and phagocytic pathways. The membrane-enclosed area of the lysosome, called the lumen, contains hydrolytic enzymes active in an acidic environment. These acid hydrolases are functional at a pH between 4.5 and 5 and are involved in cellular processes such as cell signaling, energy metabolism, restoration of the plasma membrane,...

Lysosomes

Lysosomes

Lysosomes are membrane-enclosed spherical sacs derived from the Golgi apparatus. The most important function of the lysosome is degrading macromolecules and biological polymers that are released during membrane trafficking events such as the secretory, endocytic, autophagic, and phagocytic pathways. The degradation is carried out by several hydrolytic enzymes active in an acidic environment of the lysosomal lumen. These acid hydrolases are involved in cellular processes such as cell signaling,...

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Related Experiment Video

Updated: Jul 27, 2025

Characterization of Neuronal Lysosome Interactome with Proximity Labeling Proteomics

Characterization of Neuronal Lysosome Interactome with Proximity Labeling Proteomics

Published on: June 23, 2022

Probing lysosomal activity

Xin Li^1,2, Yanan Sun¹, Xiaochen Wang^1,2

¹National Laboratory of Biomacromolecules, CAS Center for Excellence in Biomacromolecules, Institute of Biophysics, Chinese Academy of Science, Beijing 100101, China.

Biophysics Reports

|June 8, 2023

Summary

This summary is machine-generated.

Researchers developed new methods to track lysosome function in living organisms. These assays quantify lysosome maturation, acidity, and enzyme activity in C. elegans, aiding the study of cellular homeostasis.

Keywords:

Acidification C. elegans Cleavage activity Lysosomes Maturation

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Area of Science:

Cell Biology
Organelle Function
Homeostasis

Background:

Lysosomes are vital organelles responsible for breaking down and recycling cellular components.
They play a critical role in nutrient sensing and signaling, maintaining cell and tissue balance (homeostasis).
Tracking in vivo changes in lysosomal properties during development or stress is challenging.

Purpose of the Study:

To develop and validate novel in vivo assays for quantifying lysosome properties.
To enable the study of lysosomal function alterations in response to physiological changes.

Main Methods:

Utilized the model organism C. elegans for in vivo studies.
Developed specific assays to measure lysosome maturation, internal pH (acidification), and enzymatic (cleavage) activity.
Quantified these lysosomal parameters within the living organism.

Main Results:

Successfully established and implemented assays for in vivo lysosome analysis in C. elegans.
Demonstrated the capability of these assays to detect changes in lysosomal activity.
Showcased the utility of the assays for studying lysosome function during development and under stress.

Conclusions:

The developed assays provide a powerful tool for in vivo investigation of lysosome dynamics.
These methods facilitate the understanding of how lysosomal function contributes to organismal homeostasis.
The assays are applicable to studying lysosomal alterations in various biological contexts, including development and stress responses.