Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Evolutionary Relationships through Genome Comparisons02:54

Evolutionary Relationships through Genome Comparisons

5.9K
Genome comparison is one of the excellent ways to interpret the evolutionary relationships between organisms. The basic principle of genome comparison is that if two species share a common feature, it is likely encoded by the DNA sequence conserved between both species. The advent of genome sequencing technologies in the late 20th century enabled scientists to understand the concept of conservation of domains between species and helped them to deduce evolutionary relationships across diverse...
5.9K
Genomics02:02

Genomics

36.5K
Genomics is the science of genomes: it is the study of all the genetic material of an organism. In humans, the genome consists of information carried in 23 pairs of chromosomes in the nucleus, as well as mitochondrial DNA. In genomics, both coding and non-coding DNA is sequenced and analyzed. Genomics allows a better understanding of all living things, their evolution, and their diversity. It has a myriad of uses: for example, to build phylogenetic trees, to improve productivity and...
36.5K
Comparing Mitochondrial, Chloroplast, and Prokaryotic Genomes02:16

Comparing Mitochondrial, Chloroplast, and Prokaryotic Genomes

12.6K
The present-day mitochondrial and chloroplast genomes have retained some of the characteristics of their ancestral prokaryotes and also have acquired new attributes during their evolution within eukaryotic cells. Like prokaryotic genomes, mitochondrial and chloroplast genomes neither bind with histone-like proteins nor show complex packaging into chromosome-like structures, as observed in eukaryotes. Unlike mitotic cell divisions observed in eukaryotic cells, mitochondria and chloroplasts...
12.6K
Genome Annotation and Assembly03:36

Genome Annotation and Assembly

18.9K
The genome refers to all of the genetic material in an organism. It can range from a few million base pairs in microbial cells to several billion base pairs in many eukaryotic organisms. Genome assembly refers to the process of taking the DNA sequencing data and putting it all back together in a correct order to create a close representation of the original genome. This is followed by the identification of functional elements on the newly assembled genome, a process called genome annotation.
18.9K
Ribosome Profiling02:24

Ribosome Profiling

3.6K
Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...
3.6K
Proteomics01:33

Proteomics

7.5K
A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term...
7.5K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Real-World Efficacy of Dupilumab in Chinese Atopic Dermatitis Patients and Development of a Prediction Model.

Allergy·2026
Same author

Virulence of <i>Aspergillus flavus</i> and relatives using the <i>Galleria mellonella</i> model.

Virulence·2026
Same author

The Effect of Topical Ketoconazole and Topical Miconazole Nitrate in Modulating the Skin Microbiome and Mycobiome of Patients With Tinea Pedis.

Mycoses·2025
Same author

In silico search reveals the association of lichens with black yeast-like fungi in the order Chaetothyriales.

Fungal biology·2025
Same author

Disseminated cutaneous mucormycosis resembling multiple panniculitis.

International journal of dermatology·2025
Same author

Antifungal Resistance Patterns of Microsporum canis: A 27-Year MIC Study in Mainland China.

Mycoses·2025

Related Experiment Video

Updated: Jul 27, 2025

Transcriptomic Analysis of C. elegans RNA Sequencing Data Through the Tuxedo Suite on the Galaxy Project
10:19

Transcriptomic Analysis of C. elegans RNA Sequencing Data Through the Tuxedo Suite on the Galaxy Project

Published on: April 8, 2017

17.5K

Comparative analysis of whole genomes and transcriptomes of

Ruojun Wang1,2,3,4, Weixia Liu1,5, Xiao Liu1,6

  • 1Department of Dermatology and Venerology, Peking University First Hospital, Beijing, People's Republic of China.

Emerging Microbes & Infections
|June 8, 2023
PubMed
Summary

Genomic analysis of Microsporum canis revealed significant rearrangements in invasive strains, suggesting enhanced virulence and antifungal drug resistance. These findings offer insights into treating difficult dermatophyte infections.

Keywords:
DermatophytesMicrosporum canisWGSgenomicstranscriptomics

More Related Videos

Real-time Analysis of Transcription Factor Binding, Transcription, Translation, and Turnover to Display Global Events During Cellular Activation
12:54

Real-time Analysis of Transcription Factor Binding, Transcription, Translation, and Turnover to Display Global Events During Cellular Activation

Published on: March 7, 2018

13.6K
Leveraging CyVerse Resources for De Novo Comparative Transcriptomics of Underserved Non-model Organisms
10:41

Leveraging CyVerse Resources for De Novo Comparative Transcriptomics of Underserved Non-model Organisms

Published on: May 9, 2017

9.3K

Related Experiment Videos

Last Updated: Jul 27, 2025

Transcriptomic Analysis of C. elegans RNA Sequencing Data Through the Tuxedo Suite on the Galaxy Project
10:19

Transcriptomic Analysis of C. elegans RNA Sequencing Data Through the Tuxedo Suite on the Galaxy Project

Published on: April 8, 2017

17.5K
Real-time Analysis of Transcription Factor Binding, Transcription, Translation, and Turnover to Display Global Events During Cellular Activation
12:54

Real-time Analysis of Transcription Factor Binding, Transcription, Translation, and Turnover to Display Global Events During Cellular Activation

Published on: March 7, 2018

13.6K
Leveraging CyVerse Resources for De Novo Comparative Transcriptomics of Underserved Non-model Organisms
10:41

Leveraging CyVerse Resources for De Novo Comparative Transcriptomics of Underserved Non-model Organisms

Published on: May 9, 2017

9.3K

Area of Science:

  • Medical Mycology
  • Genomics
  • Infectious Diseases

Background:

  • Microsporum canis is a zoophilic dermatophyte causing superficial and invasive infections.
  • Understanding the genomic basis of M. canis virulence is crucial for effective treatment.

Purpose of the Study:

  • To compare the genomes and transcriptomes of invasive and noninvasive Microsporum canis strains.
  • To investigate the genetic factors contributing to M. canis invasiveness and antifungal resistance.

Main Methods:

  • Whole-genome sequencing and comparative genomic analysis.
  • Transcriptome sequencing (RNA-Seq) and gene expression profiling.
  • Antifungal susceptibility testing.

Main Results:

  • Invasive M. canis strains exhibited significant genomic rearrangements (translocations, inversions) and variations (SNPs, Indels) compared to noninvasive strains.
  • Invasive strains showed enrichment in pathways related to membrane components, iron/heme binding, DNA replication, and ribosome biogenesis.
  • Invasive strains displayed slightly reduced susceptibility to multiple antifungal agents, potentially indicating acquired drug resistance.

Conclusions:

  • Genomic alterations in M. canis strains correlate with invasiveness and may contribute to refractory infections.
  • Enriched gene pathways in invasive strains likely facilitate deeper tissue invasion.
  • Acquired antifungal drug resistance could be a factor in treatment failures for disseminated M. canis infections.