Antimicrobial evaluation of

Area of Science:

• Ethnobotany
• Pharmacology
• Microbiology

Background:

• Tripleurospermum callosum is traditionally used in Turkey for urinary and respiratory ailments.
• Urinary tract infections (UTIs) are a significant public health concern, often caused by resistant pathogens.

Purpose of the Study:

• To investigate the in vitro and in vivo antimicrobial activity of Tripleurospermum callosum extracts against common urinary pathogens.
• To determine the non-toxic concentrations of these extracts using C. elegans as a model organism.
• To analyze the phytochemical composition of the extracts.

Main Methods:

• Preparation of infusion, decoction, and 96% ethanol extracts from aerial parts of T. callosum.
• In vitro antimicrobial assays against Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa, Klebsiella aerogenes, and Candida albicans.
• In vivo antimicrobial assays and toxicity assessments using Caenorhabditis elegans.
• Phytochemical analysis using Liquid Chromatography-Mass Spectrometry (LC-MS/MS).

Main Results:

• Water extracts exhibited non-toxicity in C. elegans at concentrations ranging from 312 to 5000 µg/mL.
• The 96% ethanol extract was non-toxic at 312 µg/mL in C. elegans.
• The infusion extract demonstrated in vivo anti-infective effects against Gram-negative bacteria at concentrations of 312-5000 µg/mL.

Conclusions:

• Tripleurospermum callosum extracts possess promising non-toxic and anti-infective properties against urinary system pathogens.
• Further research into T. callosum could lead to novel therapeutic agents for UTIs.