Beyond iron: metal-binding activity of the
Dávid Szamosvári1, Viktoriia Savchenko1,2, Natalie Badouin3
1Faculty of Chemistry, Institute for Biological Chemistry & Centre for Microbiology and Environmental Systems Science, Department of Microbiology and Ecosystems Science, University of Vienna, Josef-Holaubek-Platz 2 (UZA II), 1090 Vienna, Austria. thomas.boettcher@univie.ac.at.
Organic & Biomolecular Chemistry
|June 8, 2023
Summary
Researchers synthesized novel Pseudomonas quinolone signal (PQS) motifs to chelate iron, finding they bind iron and other metal ions. This work expands understanding of PQS in metal ion binding and potential therapeutic applications.
Area of Science:
- Microbiology
- Medicinal Chemistry
- Biochemistry
Background:
- Pseudomonas quinolone signal (PQS) is a key quorum sensing molecule in Pseudomonas aeruginosa, regulating virulence.
- PQS also plays a role in iron acquisition by P. aeruginosa.
- The PQS structure is a privileged scaffold with potential for developing new chelating agents.
Purpose of the Study:
- To synthesize and evaluate crosslinked dimeric PQS-motifs as potential iron chelators.
- To investigate the metal ion binding properties of these novel PQS derivatives.
- To explore the metal binding capabilities of the natural PQS molecule beyond ferric iron.
Main Methods:
- Synthesis of two distinct types of crosslinked dimeric PQS-motifs.
- Characterization of metal complexes formed with synthesized PQS-motifs and natural PQS.
- Spectroscopic analysis (colorimetric and fluorescent) to detect metal ion complexation.
- Mass spectrometry to confirm the stoichiometry of metal-PQS complexes.
Main Results:
- Synthesized dimeric PQS-motifs successfully chelated ferric iron.
- These novel compounds formed colorful and fluorescent complexes with various metal ions.
- The natural PQS molecule was found to bind additional metal ions besides ferric iron.
- Mass spectrometry confirmed the stoichiometry of the formed metal complexes.
Conclusions:
- Crosslinked dimeric PQS-motifs are effective iron chelators and can bind other metal ions.
- These findings highlight the versatility of the PQS scaffold in metal ion coordination.
- The study expands the known repertoire of metal ions interacting with PQS and its derivatives, suggesting broader applications.
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