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Related Experiment Video

Updated: Jul 27, 2025

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Differential plant cell responses to

Yumin Kan1, Yanjie Zhang1, Wenhui Lin1

  • 1State Key Laboratory of Microbial Metabolism, Joint International Research Laboratory of Metabolic and Developmental Sciences, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University , Shanghai, China.

Mbio
|June 8, 2023
PubMed
Summary

Acidovorax citrulli uses two secretion systems, T3SS for plant infection and T6SS for bacterial competition. The T6SS can be engineered into an avirulent mutant to combat plant pathogens, offering a sustainable alternative to chemical pesticides.

Keywords:
Acidovorax citrulliRNA-SeqT6SSbiocontrolinterspecies competition

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Area of Science:

  • Plant pathology
  • Microbiology
  • Bacterial secretion systems

Background:

  • Acidovorax citrulli causes bacterial fruit blotch in cucurbits via its type III secretion system (T3SS).
  • The bacterium also possesses a type VI secretion system (T6SS) with antimicrobial properties.
  • The plant's response to these systems and potential crosstalk remain unclear.

Purpose of the Study:

  • To investigate plant cellular responses to A. citrulli's T3SS and T6SS.
  • To determine the role of T3SS and T6SS in A. citrulli infection and inter-bacterial competition.
  • To explore the potential of T6SS as a biocontrol agent.

Main Methods:

  • Transcriptomic analysis of plant cells during in planta infection with T3SS and T6SS.
  • Generation and characterization of T3SS-null and T6SS-active mutants.
  • In vitro and in vivo assays to assess virulence and competitive fitness.

Main Results:

  • T3SS and T6SS elicit distinct plant cellular responses, affecting pathways like phenylpropanoid biosynthesis and photosynthesis, respectively.
  • T6SS is crucial for A. citrulli survival against phyllosphere bacteria but not for plant virulence.
  • A T6SS-active, T3SS-null mutant (Acav) effectively inhibits other plant pathogens like Xanthomonas oryzae pv. oryzae.

Conclusions:

  • The T6SS of A. citrulli is nonpathogenic to plants and can be repurposed.
  • Engineered T6SS-active, avirulent A. citrulli mutants show potential as biocontrol agents against plant pathogens.
  • This strategy offers a sustainable alternative to chemical pesticides in agriculture.