Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Scott A Cunningham1, Christophe Rodriguez2,3, Paul-Louis Woerther2,3

  • 1Division of Clinical Microbiology, Department of Laboratory Medicine and Pathology, Mayo Clinic, Rochester, Minnesota, USA.

Microbiology Spectrum
|June 8, 2023
PubMed
Summary

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Comparison of blood culture contamination rates with standard practice versus two blood diversion devices at a single institution.

Journal of clinical microbiology·2026
Same author

Real-world emergence of nirsevimab resistance in breakthrough infections with respiratory syncytial virus-B: a multicentre observational study in France.

The Lancet. Microbe·2026
Same author

Contributions of whole-genome sequencing to the epidemiological monitoring of <i>Campylobacter</i> spp. in France.

Antimicrobial agents and chemotherapy·2026
Same author

<i>Legionella</i> 5S rRNA PCR melting temperature analysis discriminates high-risk species associated with disease severity.

Journal of clinical microbiology·2026
Same author

Dissemination of ESBL-producing <i>Escherichia coli</i> and carriage in the community worldwide: epidemiology, transmission pathways, molecular insights, and practical aspects.

Clinical microbiology reviews·2026
Same author

Immune checkpoint inhibitor myocarditis: a metagenomic investigation of infectious pathogens.

European heart journal·2026
Same journal

PHGDH restricts porcine reproductive and respiratory syndrome virus replication by sustaining cellular antioxidant capacity and redox homeostasis.

Microbiology spectrum·2026
Same journal

Sinomenine protects mice against <i>Staphylococcus aureus</i>-induced endometritis through inhibition of P2X7 receptor-mediated ferroptosis and inflammation.

Microbiology spectrum·2026
Same journal

Metabolic adaptations in antibiotic-resistant <i>Staphylococcus aureus</i>: understanding resistance mechanisms and enhancing antibiotic efficacy.

Microbiology spectrum·2026
Same journal

Validation of an integrated metagenomic pipeline combining optimized wet-lab processing and tiered reporting for CSF pathogen detection.

Microbiology spectrum·2026
Same journal

Chloramphenicol and tetracycline synergize with bacteriophage SeKF_13 to inactivate antimicrobial-resistant <i>Salmonella</i> Typhimurium.

Microbiology spectrum·2026
Same journal

Cell type-dependent induction of type I interferon and PARP1 activation in astrocytes and neurons during chikungunya virus infection.

Microbiology spectrum·2026
See all related articles
This summary is machine-generated.

Dual antibiotic resistance emerged in Cutibacterium avidum, a cause of orthopedic device-related infections (ODRIs). This study highlights potential limitations of using rifampin and levofloxacin for ODRI treatment, especially during debridement, antibiotic treatment, and implant retention (DAIR) procedures.

Area of Science:

  • Microbiology
  • Infectious Diseases
  • Genomics

Background:

  • Cutibacterium avidum is an increasing cause of orthopedic device-related infections (ODRIs).
  • Current treatment guidelines for C. avidum ODRI are lacking, with oral rifampin and fluoroquinolones often used post-intravenous therapy.
  • Debridement, antibiotic treatment, and implant retention (DAIR) is a common surgical approach for ODRI.

Purpose of the Study:

  • To report the in vivo emergence of dual resistance to rifampin and levofloxacin in C. avidum.
  • To identify the genetic basis for this acquired resistance.
  • To highlight potential therapeutic limitations of rifampin-levofloxacin combinations in ODRI management.

Main Methods:

  • Whole-genome sequencing of C. avidum isolates obtained before and after antibiotic treatment.
Keywords:
Cutibacterium avidumantibiotic resistancebone and joint infectionsin vivo emergence of resistancemechanisms of resistanceosteoarticular infection

Related Experiment Videos

  • Analysis of genetic mutations associated with antimicrobial resistance.

    • Main Results:

    • In vivo emergence of dual resistance to rifampin and levofloxacin in a C. avidum strain.
    • Identification of new mutations in rpoB (S446P) and gyrA (S101L) in the post-therapy isolate.
    • These mutations are associated with resistance to rifampin and fluoroquinolones, respectively.

    Conclusions:

    • The combination of oral rifampin and levofloxacin may have limitations for treating C. avidum ODRI, particularly in DAIR procedures.
    • Emergence of dual resistance necessitates re-evaluation of optimal therapeutic strategies for emerging ODRI pathogens.
    • Further research is needed to establish evidence-based treatment protocols for C. avidum ODRIs.