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The potential role of micro-RNA 125b-5p level in predicting outcome from thrombolytic therapy in patients with acute
Sara Mostafa1, Hatem Al Masry1, Mona Hussein2
1Department of Neurology, Beni-Suef University, Salah Salem Street, Beni-Suef, 62511, Egypt.
Abstract:
Several studies highlighted a significant role of specific miRNA as diagnostic and prognostic biomarkers for acute ischemic stroke. The aim of this work was to study micro-RNA 125b-5p level in patients with acute ischemic stroke in relation to stroke etiology, risk factors, severity and outcome. This case-control study was conducted on 40 patients with acute ischemic stroke eligible for receiving rt-PA and 40 age and sex matched healthy controls, Patients were submitted to neurological and radiological assessment. Functional outcome after 3 months was assessed using the modified Rankin Scale (mRS). Plasma micro-RNA 125b-5p levels were measured for both patients and control groups by quantitative real time PCR. MiRNA-125b-5p was extracted from the plasma samples then Real-time quantitative reversed transcription PCR (RT-qPCR) analysis was done. To analyze miRNA-125b-5p expression in plasma, the ∆Cq value of miRNA-125b-5p was calculated by subtracting Cq of miRNA-125b-5p from the average Cq of MiRNA RNU6B. Stroke patients had significantly higher circulating micro-RNA 125b-5p levels in comparison to healthy controls (P value = 0.01). The circulating levels of micro-RNA 125b-5p were positively correlated with stroke severity assessed by National Institutes of Health Stroke Scale (NIHSS) and infarction size. Stroke patients with poor outcome had significantly higher circulating levels of micro-RNA 125b-5p in comparison to those with good outcome (P value ≤ 0.001). The circulating levels of micro-RNA 125b-5p were significantly higher in patients who developed complications after receiving rt-PA (P value ≤ 0.001). Logistic regression model revealed that each unit increase in micro-RNA125b-5p decreased the odds of good outcome by 0.095 (95% CI 0.016-0.58, P value = 0.011). Plasma micro-RNA 125b-5p is significantly elevated is ischemic stroke patients. It is positively correlated with stroke severity and strongly associated with poor outcome and complications after thrombolytic therapy.
Insights
Elevated levels of micro-RNA 125b-5p in plasma are significantly associated with acute ischemic stroke severity and poor outcomes. This microRNA also predicts complications following thrombolytic therapy, highlighting its potential as a prognostic biomarker.
Area of Science:
- Biochemistry
- Molecular Biology
- Neurology
Background:
- MicroRNAs (miRNAs) are emerging as crucial regulators in various biological processes.
- Specific miRNAs have shown potential as diagnostic and prognostic biomarkers for acute ischemic stroke.
- Micro-RNA 125b-5p (miR-125b-5p) role in ischemic stroke requires further investigation.
Purpose of the Study:
- To investigate the plasma levels of miR-125b-5p in patients with acute ischemic stroke.
- To correlate miR-125b-5p levels with stroke etiology, risk factors, severity, and patient outcomes.
- To assess the association of miR-125b-5p with complications after rt-PA treatment.
Main Methods:
- A case-control study involving 40 acute ischemic stroke patients (rt-PA eligible) and 40 healthy controls.
- Neurological and radiological assessments were performed on patients.
- Plasma miR-125b-5p levels were quantified using quantitative real-time PCR (RT-qPCR).
- Functional outcome was assessed using the modified Rankin Scale (mRS) at 3 months.
Main Results:
- Stroke patients exhibited significantly higher circulating miR-125b-5p levels compared to healthy controls (P=0.01).
- miR-125b-5p levels positively correlated with stroke severity (NIHSS) and infarct size.
- Higher miR-125b-5p levels were observed in patients with poor outcomes (P≤0.001) and those developing rt-PA complications (P≤0.001).
- Logistic regression indicated that increased miR-125b-5p levels decreased the odds of a good outcome (P=0.011).
Conclusions:
- Plasma miR-125b-5p is significantly elevated in patients with acute ischemic stroke.
- Circulating miR-125b-5p levels are a strong indicator of stroke severity and are associated with poor functional outcomes.
- miR-125b-5p may serve as a predictive biomarker for complications following thrombolytic therapy in ischemic stroke patients.
