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Fabiana Bisaro1,2, Howard A Shuman3,4, Mario F Feldman2

  • 1Department of Biology, The City College, City University of New York, New York, NY 10031, USA.

Microbiology (Reading, England)
|June 8, 2023
PubMed
Summary
This summary is machine-generated.

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Acinetobacter baumannii secretes microcin Mcc17978, an antimicrobial peptide that targets related Acinetobacter and E. coli strains. Its activity is enhanced under low-iron conditions, suggesting a role in microbial competition during infection.

Area of Science:

  • Microbiology
  • Bacterial Pathogenesis
  • Antimicrobial Peptides

Background:

  • Acinetobacter baumannii is a multidrug-resistant pathogen prevalent in hospital settings, causing infections in immunocompromised individuals.
  • Bacteria employ various strategies for inter-bacterial competition, including the production of antimicrobial peptides like microcins.
  • Microcins are small secreted peptides with contact-independent antimicrobial activity.

Purpose of the Study:

  • To investigate the antimicrobial activity and regulation of a novel class II microcin, Mcc17978, produced by Acinetobacter baumannii ATCC 17978.
  • To identify the genetic basis and molecular targets of Mcc17978.
  • To elucidate the role of iron availability in Mcc17978 production and activity.

Main Methods:

  • Identification of the Mcc17978 genetic locus in A. baumannii ATCC 17978.
Keywords:
MicrocinAcinetobacter baumanniiFiuIronIron uptake receptorPiuA

Related Experiment Videos

  • Bacterial genetics approaches to determine Mcc17978 receptors in Escherichia coli (Fiu) and Acinetobacter (PiuA).
  • Analysis of Mcc17978 gene expression and antimicrobial activity under varying iron concentrations, including investigation of Fur binding sites.

    • Main Results:

    • Acinetobacter baumannii ATCC 17978 produces Mcc17978, a microcin active against related Acinetobacter species and surprisingly, Escherichia coli.
    • The iron-catecholate transporter Fiu in E. coli and its homolog PiuA in Acinetobacter were identified as Mcc17978 receptors.
    • Mcc17978 expression and activity are upregulated under low-iron conditions, mediated by the Ferric uptake regulator (Fur).

    Conclusions:

    • Acinetobacter baumannii utilizes Mcc17978 as a weapon for inter-bacterial competition, particularly in iron-limited host environments.
    • The identification of Fiu and PiuA as receptors provides insights into the mechanism of microcin action and host-pathogen interactions.
    • Mcc17978 represents a potential target for therapeutic strategies against A. baumannii infections.