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Related Experiment Video

Updated: Jun 15, 2026

Quantitative High-throughput Single-cell Cytotoxicity Assay For T Cells

Quantitative High-throughput Single-cell Cytotoxicity Assay For T Cells

Published on: February 2, 2013

A high-throughput cytotoxicity screening platform reveals agr-independent mutations in bacteraemia-associated

Abderrahman Hachani¹, Stefano G Giulieri¹, Romain Guérillot¹

¹Department of Microbiology and Immunology, Doherty Institute, University of Melbourne, Melbourne, Australia.

|June 8, 2023

Summary

This summary is machine-generated.

Staphylococcus aureus can hide inside host cells, causing serious infections. A new platform, InToxSa, identifies bacterial mutations that help S. aureus survive and persist within cells, offering new insights into infection persistence.

Keywords:

GWAS Staphylococcus aureus bacterial population genomics cytotoxicity evolutionary convergence analysis genetics genomics infectious disease intracellular microbiology

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High Throughput, Real-time, Dual-readout Testing of Intracellular Antimicrobial Activity and Eukaryotic Cell Cytotoxicity

High Throughput, Real-time, Dual-readout Testing of Intracellular Antimicrobial Activity and Eukaryotic Cell Cytotoxicity

Published on: November 16, 2016

Area of Science:

Microbiology
Pathogen Biology
Genomics

Background:

Staphylococcus aureus is a significant cause of mortality, often acting as an intracellular pathogen.
Intracellular S. aureus evades immune responses, leading to host cell death.
Existing methods for assessing bacterial cytotoxicity are insufficient for intracellular bacteria.

Purpose of the Study:

To develop a high-throughput cell-based platform, InToxSa, for quantifying intracellular S. aureus phenotypes.
To identify genetic mutations in clinical S. aureus isolates that influence cytotoxicity and intracellular persistence.
To understand the pathoadaptive mechanisms of S. aureus intracellular residency.

Main Methods:

Development of the InToxSa (intracellular toxicity of S. aureus) phenomics platform.
Analysis of 387 S. aureus bacteraemia isolates using comparative, statistical, and functional genomics.
Identification and characterization of mutations affecting bacterial cytotoxicity and intracellular survival.

Main Results:

The InToxSa platform successfully quantified intracellular S. aureus phenotypes.
Mutations in the Agr quorum sensing system and other loci were linked to reduced cytotoxicity and enhanced intracellular persistence.
Clinical mutations in ausA, encoding aureusimine, were found to decrease cytotoxicity and promote intracellular persistence.

Conclusions:

InToxSa is a versatile tool for studying intracellular bacterial pathogens.
The study identified novel S. aureus pathoadaptive mutations promoting intracellular residency.
Understanding these mutations can inform strategies against persistent S. aureus infections.