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Updated: Jul 27, 2025
Development and Functionalization of Electrolyte-Gated Graphene Field-Effect Transistor for Biomarker Detection
Published on: February 1, 2022
Preparation of self-supporting vertically/horizontally grown graphene microelectrodes for neurotransmitter
Yujie Xue1, Hongji Li2, Xiaoyan Wang2
1Tianjin Key Laboratory of Film Electronic and Communication Devices, School of Integrated Circuit Science and Engineering, Tianjin University of Technology, Tianjin, 300384, PR China.
Abstract:
The development of microelectrodes for the rapid in situ detection of neurotransmitters and their metabolic levels in human biofluids has considerable significance in biomedical research. In this study, self-supported graphene microelectrodes with B-doped, N-doped, and B- and N-co-doped vertical graphene (BVG, NVG, and BNVG, respectively) nanosheets grown on horizontal graphene (HG) were fabricated for the first time. The high electrochemical catalytic activity of BVG/HG on monoamine compounds was explored by investigating the influence of B and N atoms and the VG layer thickness on the response current of neurotransmitters. Quantitative analysis using the BVG/HG electrode in a blood-like environment with pH 7.4 indicated that the linear concentration ranges were 1-400 and 1-350 μM for dopamine (DA) and serotonin (5-HT), with limits of detection (LODs) of 0.271 and 0.361 μM, respectively. For tryptophan (Trp), the sensor measured a wide linear concentration range of 3-1500 μM over a wide pH range of 5.0-9.0, with the LOD fluctuating between 0.58 and 1.04 μM. Furthermore, the BVG/HG microelectrodes could be developed as needle- and pen-type sensors for the detection of DA, 5-HT, and Trp in human blood and gastrointestinal secretion samples.
