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Related Experiment Video

Updated: Jul 27, 2025

Design and Validation of a Volumetric-extrusion Bioprinter for Bioprinting of Soluble Basement Membrane Extract for Translational Research
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Ti

Mengxi Chen1, Qi Zhang1, Fang Xu1

  • 1College of Pharmaceutical Sciences, Soochow University, Suzhou, 215123, China.

Analytica Chimica Acta
|June 8, 2023
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Summary

MXene materials efficiently isolate extracellular vesicles (EVs) from biological samples. This novel coprecipitation method offers a rapid, cost-effective tool for disease biomarker discovery and early diagnosis.

Keywords:
Affinity chromatographyBiomarkersCoprecipitationExtracellular vesiclesMXene

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Area of Science:

  • Materials Science
  • Biotechnology
  • Nanotechnology

Background:

  • Two-dimensional (2D) materials like MXenes are known for catalysis and battery applications due to surface properties.
  • Extracellular vesicles (EVs) are crucial biomarkers for disease detection and monitoring treatment response.
  • The application of MXenes in biological sample treatment, particularly for EV isolation, remains underexplored.

Purpose of the Study:

  • To synthesize and evaluate MXene materials (Ti3C2 and Ti2C) for efficient isolation of extracellular vesicles (EVs) from biological samples.
  • To investigate the affinity interaction between titanium in MXenes and the phospholipid membrane of EVs for optimized isolation.
  • To establish a rapid, cost-effective method for EV isolation suitable for downstream proteomic and RNA analysis.

Main Methods:

  • Synthesis of Ti3C2 and Ti2C MXene materials.
  • Isolation of EVs from biological samples using MXene materials via affinity interaction and coprecipitation.
  • Comparison of Ti3C2 MXene performance against Ti2C MXene, TiO2 beads, and other isolation methods.
  • Analysis of isolated EVs for protein and ribonucleic acid (RNA) content.
  • Proteomics analysis of EVs isolated from colorectal cancer (CRC) patient blood plasma.

Main Results:

  • Ti3C2 MXene demonstrated superior EVs isolation performance compared to Ti2C MXene, TiO2 beads, and other methods.
  • The Ti3C2 MXene method required the lowest material dosage and completed isolation within 30 minutes.
  • The isolation process is convenient, economical, and compatible with subsequent protein and RNA analyses.
  • Proteomics analysis of EVs from CRC patients revealed 67 upregulated proteins associated with CRC progression.

Conclusions:

  • MXene material-based EVs isolation via coprecipitation is a highly efficient method.
  • This technique provides a valuable tool for the early diagnosis of diseases like colorectal cancer.
  • The rapid and cost-effective nature of this method facilitates biomarker discovery and clinical applications.